TY - JOUR
T1 - Structure of the Aeropyrum pernix L7Ae multifunctional protein and insight into its extreme thermostability
AU - Bhuiya, Mohammad Wadud
AU - Suryadi, Jimmy
AU - Zhou, Zholi
AU - Brown, Bernard Andrew
PY - 2013/9
Y1 - 2013/9
N2 - Archaeal ribosomal protein L7Ae is a multifunctional RNA-binding protein that directs post-transcriptional modification of archaeal RNAs. The L7Ae protein from Aeropyrum pernix (Ap L7Ae), a member of the Crenarchaea, was found to have an extremely high melting temperature (>383K). The crystal structure of Ap L7Ae has been determined to a resolution of 1.56Å. The structure of Ap L7Ae was compared with the structures of two homologs: hyperthermophilic Methanocaldococcus jannaschii L7Ae and the mesophilic counterpart mammalian 15.5kD protein. The primary stabilizing feature in the Ap L7Ae protein appears to be the large number of ion pairs and extensive ion-pair network that connects secondary-structural elements. To our knowledge, Ap L7Ae is among the most thermostable single-domain monomeric proteins presently observed.
AB - Archaeal ribosomal protein L7Ae is a multifunctional RNA-binding protein that directs post-transcriptional modification of archaeal RNAs. The L7Ae protein from Aeropyrum pernix (Ap L7Ae), a member of the Crenarchaea, was found to have an extremely high melting temperature (>383K). The crystal structure of Ap L7Ae has been determined to a resolution of 1.56Å. The structure of Ap L7Ae was compared with the structures of two homologs: hyperthermophilic Methanocaldococcus jannaschii L7Ae and the mesophilic counterpart mammalian 15.5kD protein. The primary stabilizing feature in the Ap L7Ae protein appears to be the large number of ion pairs and extensive ion-pair network that connects secondary-structural elements. To our knowledge, Ap L7Ae is among the most thermostable single-domain monomeric proteins presently observed.
KW - Aeropyrum pernix
KW - L7Ae
KW - ion pairs
KW - ribosomal proteins
KW - thermal stability
UR - http://www.scopus.com/inward/record.url?scp=84883384446&partnerID=8YFLogxK
U2 - 10.1107/S1744309113021799
DO - 10.1107/S1744309113021799
M3 - Article
C2 - 23989144
AN - SCOPUS:84883384446
SN - 1744-3091
VL - 69
SP - 979
EP - 988
JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
IS - 9
ER -