M7GHub: Deciphering the location, regulation and pathogenesis of internal mRNA N7-methylguanosine (m7G) sites in human

Bowen Song, Yujiao Tang, Kunqi Chen, Zhen Wei, Rong Rong, Zhiliang Lu, Jionglong Su, João Pedro De Magalhães*, Daniel J. Rigden, Meng Jia

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

95 Citations (Scopus)

Abstract

Motivation: Recent progress in N7-methylguanosine (m7G) RNA methylation studies has focused on its internal (rather than capped) presence within mRNAs. Tens of thousands of internal mRNA m7G sites have been identified within mammalian transcriptomes, and a single resource to best share, annotate and analyze the massive m7G data generated recently are sorely needed. Results: We report here m7GHub, a comprehensive online platform for deciphering the location, regulation and pathogenesis of internal mRNA m7G. The m7GHub consists of four main components, including: the first internal mRNA m7G database containing 44 058 experimentally validated internal mRNA m7G sites, a sequence-based highaccuracy predictor, the first web server for assessing the impact of mutations on m7G status, and the first database recording 1218 disease-associated genetic mutations that may function through regulation of m7G methylation. Together, m7GHub will serve as a useful resource for research on internal mRNA m7G modification. Availability and implementation: m7GHub is freely accessible online at www.xjtlu.edu.cn/biologicalsciences/ m7ghub.

Original languageEnglish
Pages (from-to)3528-3536
Number of pages9
JournalBioinformatics
Volume36
Issue number11
DOIs
Publication statusPublished - 1 Jun 2020

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