Estimate the proportion of non-specific binding in MeRIP-Seq data

Jingxuan Bao; Zhen Wei; Jia Meng; Jionglong Su

Estimate the proportion of non-specific binding in MeRIP-Seq data

Jingxuan Bao, Zhen Wei, Jia Meng, Jionglong Su^*

^*Corresponding author for this work

Xi'an Jiaotong-Liverpool University

Research output: Chapter in Book or Report/Conference proceeding › Conference Proceeding › peer-review

Abstract

The ISOpureR method uses the gradient decent method to maximize the complete likelihood function of estimating the proper estimators. In this research, we estimate the absolute proportion of m6A specific binding mRNA in the m6Acontaining sample (IP sample) with the application of ISOpureR method. We apply the ISOpureR method to simulated data and real data. Our results show that the method performs well under the model of second order median regression in terms of the simulated data with application of regression analysis; and it needs to be further developed when applied to the real data.

Original language	English
Title of host publication	3rd International Conference on Biological Information and Biomedical Engineering, BIBE 2019
Publisher	VDE Verlag GmbH
Pages	392-396
Number of pages	5
ISBN (Electronic)	9783800750269
Publication status	Published - 2019
Event	3rd International Conference on Biological Information and Biomedical Engineering, BIBE 2019 - Hangzhou, China Duration: 20 Jul 2019 → 22 Jul 2019

Publication series

Name	3rd International Conference on Biological Information and Biomedical Engineering, BIBE 2019

Conference

Conference	3rd International Conference on Biological Information and Biomedical Engineering, BIBE 2019
Country/Territory	China
City	Hangzhou
Period	20/07/19 → 22/07/19

Cite this

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title = "Estimate the proportion of non-specific binding in MeRIP-Seq data",

abstract = "The ISOpureR method uses the gradient decent method to maximize the complete likelihood function of estimating the proper estimators. In this research, we estimate the absolute proportion of m6A specific binding mRNA in the m6Acontaining sample (IP sample) with the application of ISOpureR method. We apply the ISOpureR method to simulated data and real data. Our results show that the method performs well under the model of second order median regression in terms of the simulated data with application of regression analysis; and it needs to be further developed when applied to the real data.",

author = "Jingxuan Bao and Zhen Wei and Jia Meng and Jionglong Su",

note = "Publisher Copyright: {\textcopyright} VDE VERLAG GMBH · Berlin · Offenbach.; 3rd International Conference on Biological Information and Biomedical Engineering, BIBE 2019 ; Conference date: 20-07-2019 Through 22-07-2019",

year = "2019",

language = "English",

series = "3rd International Conference on Biological Information and Biomedical Engineering, BIBE 2019",

publisher = "VDE Verlag GmbH",

pages = "392--396",

booktitle = "3rd International Conference on Biological Information and Biomedical Engineering, BIBE 2019",

}

Bao, J, Wei, Z , Meng, J & Su, J 2019, Estimate the proportion of non-specific binding in MeRIP-Seq data. in 3rd International Conference on Biological Information and Biomedical Engineering, BIBE 2019. 3rd International Conference on Biological Information and Biomedical Engineering, BIBE 2019, VDE Verlag GmbH, pp. 392-396, 3rd International Conference on Biological Information and Biomedical Engineering, BIBE 2019, Hangzhou, China, 20/07/19.

Estimate the proportion of non-specific binding in MeRIP-Seq data. / Bao, Jingxuan; Wei, Zhen ; Meng, Jia et al.
3rd International Conference on Biological Information and Biomedical Engineering, BIBE 2019. VDE Verlag GmbH, 2019. p. 392-396 (3rd International Conference on Biological Information and Biomedical Engineering, BIBE 2019).

Research output: Chapter in Book or Report/Conference proceeding › Conference Proceeding › peer-review

TY - GEN

T1 - Estimate the proportion of non-specific binding in MeRIP-Seq data

AU - Bao, Jingxuan

AU - Wei, Zhen

AU - Meng, Jia

AU - Su, Jionglong

PY - 2019

Y1 - 2019

N2 - The ISOpureR method uses the gradient decent method to maximize the complete likelihood function of estimating the proper estimators. In this research, we estimate the absolute proportion of m6A specific binding mRNA in the m6Acontaining sample (IP sample) with the application of ISOpureR method. We apply the ISOpureR method to simulated data and real data. Our results show that the method performs well under the model of second order median regression in terms of the simulated data with application of regression analysis; and it needs to be further developed when applied to the real data.

AB - The ISOpureR method uses the gradient decent method to maximize the complete likelihood function of estimating the proper estimators. In this research, we estimate the absolute proportion of m6A specific binding mRNA in the m6Acontaining sample (IP sample) with the application of ISOpureR method. We apply the ISOpureR method to simulated data and real data. Our results show that the method performs well under the model of second order median regression in terms of the simulated data with application of regression analysis; and it needs to be further developed when applied to the real data.

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M3 - Conference Proceeding

AN - SCOPUS:85096592829

T3 - 3rd International Conference on Biological Information and Biomedical Engineering, BIBE 2019

SP - 392

EP - 396

BT - 3rd International Conference on Biological Information and Biomedical Engineering, BIBE 2019

PB - VDE Verlag GmbH

T2 - 3rd International Conference on Biological Information and Biomedical Engineering, BIBE 2019

Y2 - 20 July 2019 through 22 July 2019

ER -

Estimate the proportion of non-specific binding in MeRIP-Seq data

Abstract

Publication series

Conference

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