Abstract
Development for new solid substrates for protein adsorption is important, given the massive potential in a number of fields including food production, medicine, biotechnology, and pharmaceutical processing. We developed a zeolitic imidazolate framework 8 (ZIF-8)-nanoparticles based fibrous platform (Platform 1). Its ability in protein adsorption is proven by fluorescence study of Alexa Fluor 647-labeled donkey anti-rabbit IgG adsorbed on Platform 1. Studies reveal that Platform 1 shows much more enhanced protein adsorption compared to a polyethylene terephthalate gauze fibrous platform treated with ZIF-8 nanoparticles. This may be explained by considering that more active sites are enabled on fabric to adsorb a higher amount of ZIF-8 nanoparticles. We show that Platform 1 provides favorable biocompatibility to maintain the bioactivity of enzymes. Furthermore, we prove that a carboxymethylated cotton fabric platform treated with ZIF-8 (Platform 3) can be more capable of immobilizing the Alexa Fluor 647-labeled antibodies than Platform 1. Carboxymethylation helps to enhance protein adsorption in these cotton fabric substrates.
| Original language | English |
|---|---|
| Pages (from-to) | 7626-7636 |
| Number of pages | 11 |
| Journal | ACS Applied Nano Materials |
| Volume | 2 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 27 Dec 2019 |
Keywords
- MOFs
- biocompatibility
- carboxymethylation
- protein adsorption
- substrates
