Differentiation potential of bone marrow mesenchymal stem cells into retina in normal and laser-injured rat eye

Jie Zhang; Qing Shan; Ping Ma; Yanming Jiang; Peng Chen; Jingxia Wen; You Zhou; Huanwen Qian; Xuetao Pei

doi:10.1360/03yc0081

Differentiation potential of bone marrow mesenchymal stem cells into retina in normal and laser-injured rat eye

Jie Zhang, Qing Shan, Ping Ma, Yanming Jiang, Peng Chen, Jingxia Wen, You Zhou, Huanwen Qian, Xuetao Pei^*

^*Corresponding author for this work

Academy of Military Medical Science China

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Bone marrow mesenchymal stem cells (MSCs) can develop into hematopoietic and mesenchymal lineages but have not been known to participate in the production of retina. Here we report that bone marrow mesenchymal stem cells, after being subretinally transplanted into normal or Nd: YAG laser-injured rat eye, can integrate into RPE layer, photoreceptor layer, bipolar cell layer and ganglion layer. DAPI-labeling detection was used to trace the origin of the repopulating cells. DAPI fluorescence was used to identify retina cells of bone marrow origin 10, 20, 35 and 50 days after transplantation. No formation of rosettes was found but some random cells were found at the end of the observation. MSCs-originated cells spread more widely in the injured retinas than in the normal ones. Immunohistochemical detection showed that though the cells could express neuronal nuclei (NeuN), neuron specific enolase (NSE), glial fibrillary acidic protein (GFAP) and cytokeratin (CK), the proteins expression in the injured transplantation group was abnormal in some region compared with that in the normal transplantation group. Electroretinogram (ERG) showed that ERG-b wave of the injured transplantation group is significantly higher than that of the two laser-injured control groups. These results suggest that a proportion of MSCs can differentiate into retina-like structure in vivo and the differentiation differs in normal and laser-injured retinas. Copyright by Science in China Press 2004.

Original language	English
Pages (from-to)	241-250
Number of pages	10
Journal	Science in China, Series C: Life Sciences
Volume	47
Issue number	3
DOIs	https://doi.org/10.1360/03yc0081
Publication status	Published - 2004
Externally published	Yes

Keywords

Differentiation
Laser
MSCs
Retina

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1360/03yc0081

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@article{083c396f2c774062b24f9e098dc34f66,

title = "Differentiation potential of bone marrow mesenchymal stem cells into retina in normal and laser-injured rat eye",

abstract = "Bone marrow mesenchymal stem cells (MSCs) can develop into hematopoietic and mesenchymal lineages but have not been known to participate in the production of retina. Here we report that bone marrow mesenchymal stem cells, after being subretinally transplanted into normal or Nd: YAG laser-injured rat eye, can integrate into RPE layer, photoreceptor layer, bipolar cell layer and ganglion layer. DAPI-labeling detection was used to trace the origin of the repopulating cells. DAPI fluorescence was used to identify retina cells of bone marrow origin 10, 20, 35 and 50 days after transplantation. No formation of rosettes was found but some random cells were found at the end of the observation. MSCs-originated cells spread more widely in the injured retinas than in the normal ones. Immunohistochemical detection showed that though the cells could express neuronal nuclei (NeuN), neuron specific enolase (NSE), glial fibrillary acidic protein (GFAP) and cytokeratin (CK), the proteins expression in the injured transplantation group was abnormal in some region compared with that in the normal transplantation group. Electroretinogram (ERG) showed that ERG-b wave of the injured transplantation group is significantly higher than that of the two laser-injured control groups. These results suggest that a proportion of MSCs can differentiate into retina-like structure in vivo and the differentiation differs in normal and laser-injured retinas. Copyright by Science in China Press 2004.",

keywords = "Differentiation, Laser, MSCs, Retina",

author = "Jie Zhang and Qing Shan and Ping Ma and Yanming Jiang and Peng Chen and Jingxia Wen and You Zhou and Huanwen Qian and Xuetao Pei",

note = "Funding Information: Acknowledgements We thank Dr. Peterson for the advice on in situ hybridization, Dr. Gao Yabing, Dr. Cui Xuemei (Department of Radiopathology, Beijing Institute of Radiation Medicine ) for the tissue section, Dr. Wang Gang (No. 302 Hospital) for the PCR instruction and Dr. Dong Bo (Department of Roentgenopathy Therapy, Beijing Institute of Radiation Medicine) for flow cytometry identification of MSCs. This work was supported by the Chinese National Key Project of Basic Research (Grant No. 2001CB509906) and State 863 High Technology R&D Project of China (Grant Nos. 2002AA205051 & 2003AA205160).",

year = "2004",

doi = "10.1360/03yc0081",

language = "English",

volume = "47",

pages = "241--250",

journal = "Science in China, Series C: Life Sciences",

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TY - JOUR

T1 - Differentiation potential of bone marrow mesenchymal stem cells into retina in normal and laser-injured rat eye

AU - Zhang, Jie

AU - Shan, Qing

AU - Ma, Ping

AU - Jiang, Yanming

AU - Chen, Peng

AU - Wen, Jingxia

AU - Zhou, You

AU - Qian, Huanwen

AU - Pei, Xuetao

N1 - Funding Information: Acknowledgements We thank Dr. Peterson for the advice on in situ hybridization, Dr. Gao Yabing, Dr. Cui Xuemei (Department of Radiopathology, Beijing Institute of Radiation Medicine ) for the tissue section, Dr. Wang Gang (No. 302 Hospital) for the PCR instruction and Dr. Dong Bo (Department of Roentgenopathy Therapy, Beijing Institute of Radiation Medicine) for flow cytometry identification of MSCs. This work was supported by the Chinese National Key Project of Basic Research (Grant No. 2001CB509906) and State 863 High Technology R&D Project of China (Grant Nos. 2002AA205051 & 2003AA205160).

PY - 2004

Y1 - 2004

N2 - Bone marrow mesenchymal stem cells (MSCs) can develop into hematopoietic and mesenchymal lineages but have not been known to participate in the production of retina. Here we report that bone marrow mesenchymal stem cells, after being subretinally transplanted into normal or Nd: YAG laser-injured rat eye, can integrate into RPE layer, photoreceptor layer, bipolar cell layer and ganglion layer. DAPI-labeling detection was used to trace the origin of the repopulating cells. DAPI fluorescence was used to identify retina cells of bone marrow origin 10, 20, 35 and 50 days after transplantation. No formation of rosettes was found but some random cells were found at the end of the observation. MSCs-originated cells spread more widely in the injured retinas than in the normal ones. Immunohistochemical detection showed that though the cells could express neuronal nuclei (NeuN), neuron specific enolase (NSE), glial fibrillary acidic protein (GFAP) and cytokeratin (CK), the proteins expression in the injured transplantation group was abnormal in some region compared with that in the normal transplantation group. Electroretinogram (ERG) showed that ERG-b wave of the injured transplantation group is significantly higher than that of the two laser-injured control groups. These results suggest that a proportion of MSCs can differentiate into retina-like structure in vivo and the differentiation differs in normal and laser-injured retinas. Copyright by Science in China Press 2004.

AB - Bone marrow mesenchymal stem cells (MSCs) can develop into hematopoietic and mesenchymal lineages but have not been known to participate in the production of retina. Here we report that bone marrow mesenchymal stem cells, after being subretinally transplanted into normal or Nd: YAG laser-injured rat eye, can integrate into RPE layer, photoreceptor layer, bipolar cell layer and ganglion layer. DAPI-labeling detection was used to trace the origin of the repopulating cells. DAPI fluorescence was used to identify retina cells of bone marrow origin 10, 20, 35 and 50 days after transplantation. No formation of rosettes was found but some random cells were found at the end of the observation. MSCs-originated cells spread more widely in the injured retinas than in the normal ones. Immunohistochemical detection showed that though the cells could express neuronal nuclei (NeuN), neuron specific enolase (NSE), glial fibrillary acidic protein (GFAP) and cytokeratin (CK), the proteins expression in the injured transplantation group was abnormal in some region compared with that in the normal transplantation group. Electroretinogram (ERG) showed that ERG-b wave of the injured transplantation group is significantly higher than that of the two laser-injured control groups. These results suggest that a proportion of MSCs can differentiate into retina-like structure in vivo and the differentiation differs in normal and laser-injured retinas. Copyright by Science in China Press 2004.

KW - Differentiation

KW - Laser

KW - MSCs

KW - Retina

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DO - 10.1360/03yc0081

M3 - Article

C2 - 15524281

AN - SCOPUS:16644381437

SN - 1006-9305

VL - 47

SP - 241

EP - 250

JO - Science in China, Series C: Life Sciences

JF - Science in China, Series C: Life Sciences

IS - 3

ER -

Differentiation potential of bone marrow mesenchymal stem cells into retina in normal and laser-injured rat eye

Abstract

Keywords

UN SDGs

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