TY - GEN
T1 - An automated microfluidic system for high-speed, high accuracy size synchronization of caenorhabditis elegans
AU - Song, P. F.
AU - Dong, X. K.
AU - Liu, X. Y.
N1 - Publisher Copyright:
© 15CBMS-0001.
PY - 2015
Y1 - 2015
N2 - This paper reports a novel microfluidic system that enables, for the first time, automated, high-speed and high-accuracy size synchronization of the nematode worm Caenorhabditis elegans. The microfluidic device, integrated with a real-time image processing algorithm, is capable of sequentially loading, realtime size measurement, and size-based sorting of C. elegans. Using this device, we demonstrate a sorting speed of 11 worm/min with a zero false positive rate and a 3.1% (1/32) false negative rate. The superior performance provided by the microfluidic system will significantly facilitate large-scale size synchronization of C. elegans.
AB - This paper reports a novel microfluidic system that enables, for the first time, automated, high-speed and high-accuracy size synchronization of the nematode worm Caenorhabditis elegans. The microfluidic device, integrated with a real-time image processing algorithm, is capable of sequentially loading, realtime size measurement, and size-based sorting of C. elegans. Using this device, we demonstrate a sorting speed of 11 worm/min with a zero false positive rate and a 3.1% (1/32) false negative rate. The superior performance provided by the microfluidic system will significantly facilitate large-scale size synchronization of C. elegans.
KW - Automation
KW - C. elegans
KW - Microfluidics
KW - Size synchronization
UR - http://www.scopus.com/inward/record.url?scp=84983242546&partnerID=8YFLogxK
M3 - Conference Proceeding
AN - SCOPUS:84983242546
T3 - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 719
EP - 721
BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Y2 - 25 October 2015 through 29 October 2015
ER -