TY - GEN
T1 - A Paper-Based Microfluidic Analytical Device with A Highly Integrated On-Chip Valve for Autonomous ELISA
AU - Cai, Tianyu
AU - Duan, Sixuan
AU - Fu, Hao
AU - Zhu, Jia
AU - Lim, Eng Gee
AU - Huang, Kaizhu
AU - Hoettges, Kai
AU - Liu, Xinyu
AU - Song, Pengfei
N1 - Funding Information:
T. Cai and S. Duan contributed equally to this work. The authors thank the financial support from the programs of 2020 Natural Science Foundation of the Jiangsu Higher Education (20KJB460024), Jiangsu Science and Technology Programme - Young Scholar (SBK2020041995), Jiangsu Province High-level Innovation and Entrepreneurship Talent Plan (2020-30803), XJTLU Key Programme Special Fund – Exploratory Research Programme (KSF-E-39), and XJTLU Research Development Fund (RDF-18-02-20). The authors also acknowledge the financial support from Xi’an Jiaotong – Liverpool University to S. Duan (PGRS1912019).
Publisher Copyright:
© 2022 IEEE.
PY - 2022
Y1 - 2022
N2 - This paper reports a microfluidic paper-based analytical device (PAD), featuring a novel, highly integrated on-chip rotary valve, for autonomous enzyme-linked immunosorbent assay (ELISA). Unlike existing valving methods on PADs, this rotary valve allows regulation of multiple flows with a single valve, leading to ease of assembly and small footprint with a high integration level. The micromotor-controlled valve is also programmable and highly reliable, and has a fast response (76 ± 0.6ms, n=20, 500 times faster than previous smart material-enabled valves on PADs). Using this design, we performed autonomous ELISA, demonstrating a limit of detection (LOD) of 20 pM for rabbit IgG antibody detection with 100% valving reliability.
AB - This paper reports a microfluidic paper-based analytical device (PAD), featuring a novel, highly integrated on-chip rotary valve, for autonomous enzyme-linked immunosorbent assay (ELISA). Unlike existing valving methods on PADs, this rotary valve allows regulation of multiple flows with a single valve, leading to ease of assembly and small footprint with a high integration level. The micromotor-controlled valve is also programmable and highly reliable, and has a fast response (76 ± 0.6ms, n=20, 500 times faster than previous smart material-enabled valves on PADs). Using this design, we performed autonomous ELISA, demonstrating a limit of detection (LOD) of 20 pM for rabbit IgG antibody detection with 100% valving reliability.
KW - Microfluidic paper-based analytical device
KW - autonomous ELISA
KW - in-vitro diagnostics
KW - on-chip valve
UR - http://www.scopus.com/inward/record.url?scp=85126394524&partnerID=8YFLogxK
U2 - 10.1109/MEMS51670.2022.9699652
DO - 10.1109/MEMS51670.2022.9699652
M3 - Conference Proceeding
AN - SCOPUS:85126394524
SP - 271
EP - 274
BT - 35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 35th IEEE International Conference on Micro Electro Mechanical Systems Conference, MEMS 2022
Y2 - 9 January 2022 through 13 January 2022
ER -