A microfluidic device for Caenorhabditis elegans based chemical testing

Pengfei Song; Weize Zhang; Alexandre Sobolevski; Kristine Bernard; Siegfried Hekimi; Xinyu Liu

doi:10.1115/IMECE2014-39126

A microfluidic device for Caenorhabditis elegans based chemical testing

Pengfei Song, Weize Zhang, Alexandre Sobolevski, Kristine Bernard, Siegfried Hekimi, Xinyu Liu^*

^*Corresponding author for this work

McGill University

Research output: Contribution to conference › Paper › peer-review

Abstract

The nematode worm Caenorhabditis elegans has been employed as a popular model organism in many fields of biological research. In this paper, we present an easy-to-use microfluidic device for facilitating C. elegans based chemical testing. The device is capable of housing single worms in microfluidic chambers and precisely adjusting the chamber's chemical environment during experiments. Eight worms can be readily loaded into the chambers through separate loading channels in a quick and gentle manner. In addition, a custom-made software with a graphic user interface is also created for quantitative analysis of locomotion parameters (swimming frequency and bend amplitude) of the worms in response to chemical stimuli, thus greatly enhancing the efficiency of data collection. We perform proof-ofconcept experiments using two chemicals, zinc ion (Zn²⁺) and glucose, which demonstrate the capability and effectiveness of the microfluidic device.

Original language	English
DOIs	https://doi.org/10.1115/IMECE2014-39126
Publication status	Published - 2014
Externally published	Yes
Event	ASME 2014 International Mechanical Engineering Congress and Exposition, IMECE 2014 - Montreal, Canada Duration: 14 Nov 2014 → 20 Nov 2014

Conference

Conference	ASME 2014 International Mechanical Engineering Congress and Exposition, IMECE 2014
Country/Territory	Canada
City	Montreal
Period	14/11/14 → 20/11/14

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title = "A microfluidic device for Caenorhabditis elegans based chemical testing",

abstract = "The nematode worm Caenorhabditis elegans has been employed as a popular model organism in many fields of biological research. In this paper, we present an easy-to-use microfluidic device for facilitating C. elegans based chemical testing. The device is capable of housing single worms in microfluidic chambers and precisely adjusting the chamber's chemical environment during experiments. Eight worms can be readily loaded into the chambers through separate loading channels in a quick and gentle manner. In addition, a custom-made software with a graphic user interface is also created for quantitative analysis of locomotion parameters (swimming frequency and bend amplitude) of the worms in response to chemical stimuli, thus greatly enhancing the efficiency of data collection. We perform proof-ofconcept experiments using two chemicals, zinc ion (Zn2+) and glucose, which demonstrate the capability and effectiveness of the microfluidic device.",

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AU - Song, Pengfei

AU - Zhang, Weize

AU - Sobolevski, Alexandre

AU - Bernard, Kristine

AU - Hekimi, Siegfried

AU - Liu, Xinyu

PY - 2014

Y1 - 2014

N2 - The nematode worm Caenorhabditis elegans has been employed as a popular model organism in many fields of biological research. In this paper, we present an easy-to-use microfluidic device for facilitating C. elegans based chemical testing. The device is capable of housing single worms in microfluidic chambers and precisely adjusting the chamber's chemical environment during experiments. Eight worms can be readily loaded into the chambers through separate loading channels in a quick and gentle manner. In addition, a custom-made software with a graphic user interface is also created for quantitative analysis of locomotion parameters (swimming frequency and bend amplitude) of the worms in response to chemical stimuli, thus greatly enhancing the efficiency of data collection. We perform proof-ofconcept experiments using two chemicals, zinc ion (Zn2+) and glucose, which demonstrate the capability and effectiveness of the microfluidic device.

AB - The nematode worm Caenorhabditis elegans has been employed as a popular model organism in many fields of biological research. In this paper, we present an easy-to-use microfluidic device for facilitating C. elegans based chemical testing. The device is capable of housing single worms in microfluidic chambers and precisely adjusting the chamber's chemical environment during experiments. Eight worms can be readily loaded into the chambers through separate loading channels in a quick and gentle manner. In addition, a custom-made software with a graphic user interface is also created for quantitative analysis of locomotion parameters (swimming frequency and bend amplitude) of the worms in response to chemical stimuli, thus greatly enhancing the efficiency of data collection. We perform proof-ofconcept experiments using two chemicals, zinc ion (Zn2+) and glucose, which demonstrate the capability and effectiveness of the microfluidic device.

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A microfluidic device for Caenorhabditis elegans based chemical testing

Abstract

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