TY - JOUR
T1 - Transcriptome analysis of potential flocculation-related genes in Streptomyces sp. hsn06 with flocculation activity on Chlorella vulgaris biomass
AU - Li, Yi
AU - Ma, Muyuan
AU - Jing, Ruxian
AU - Zhang, Zhiyu
AU - Jiang, Xiaobing
AU - Wang, Hailei
N1 - Funding Information:
This work is supported by National Science Foundation of China (32000073), Natural Science Foundation of Henan Province (202300410240), and the Key Scientific Research Programs of Henan Education Department (21A180011).
Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2022/1
Y1 - 2022/1
N2 - Chlorella vulgaris is a biomass energy provider with promising potential to help alleviate the energy crisis. Streptomyces sp. hsn06, as an actinomycete, can harvest C. vulgaris biomass safely and efficiently through flocculation activity, and proteins contribute greatly to the flocculation effect. However, potential flocculation protein-related genes are unclear. The mycelia of strain hsn06 after culture with glucose as the sole carbon source exhibited significantly higher flocculation activity as well as higher protein contents than those cultured with starch as the carbon source. To further explore the flocculation mechanism, the mycelia of strain hsn06 with distinct flocculation activities after culture with different carbon sources were examined by transcriptome analysis. We found that 403 genes were differentially up-regulated in mycelia cultured with glucose, compared to those cultured with starch as the carbon source. Five significantly differentially expressed protein-related genes were determined and confirmed by qRT-PCR, which indicated that three of the selected genes were potential flocculation-related genes. These results advance our understanding of potential flocculation-related genes during the harvesting of microalgal biomass.
AB - Chlorella vulgaris is a biomass energy provider with promising potential to help alleviate the energy crisis. Streptomyces sp. hsn06, as an actinomycete, can harvest C. vulgaris biomass safely and efficiently through flocculation activity, and proteins contribute greatly to the flocculation effect. However, potential flocculation protein-related genes are unclear. The mycelia of strain hsn06 after culture with glucose as the sole carbon source exhibited significantly higher flocculation activity as well as higher protein contents than those cultured with starch as the carbon source. To further explore the flocculation mechanism, the mycelia of strain hsn06 with distinct flocculation activities after culture with different carbon sources were examined by transcriptome analysis. We found that 403 genes were differentially up-regulated in mycelia cultured with glucose, compared to those cultured with starch as the carbon source. Five significantly differentially expressed protein-related genes were determined and confirmed by qRT-PCR, which indicated that three of the selected genes were potential flocculation-related genes. These results advance our understanding of potential flocculation-related genes during the harvesting of microalgal biomass.
KW - Chlorella vulgaris biomass
KW - Potential flocculation-related genes
KW - qRT-PCR
KW - Streptomyces sp. hsn06
KW - Transcriptome analysis
UR - http://www.scopus.com/inward/record.url?scp=85121495718&partnerID=8YFLogxK
U2 - 10.1007/s00203-021-02647-2
DO - 10.1007/s00203-021-02647-2
M3 - Article
C2 - 34932151
AN - SCOPUS:85121495718
SN - 0302-8933
VL - 204
JO - Archives of Microbiology
JF - Archives of Microbiology
IS - 1
M1 - 41
ER -