Protocol for generating human induced neural progenitor cells from immobilized adult peripheral blood

Ting Zhang, Ran Tao, Chunmei Yue*, Naihe Jing*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Generating induced neural stem/progenitor cells (iNPCs) from somatic cells for medical applications has remained challenging. Here, we describe a reliable protocol to make human iNPCs from a small volume of immobilized adult peripheral blood by direct reprogramming. We have verified that the integration-free human iNPCs can efficiently differentiate into mature neurons in mouse brain upon transplantation and display capacities to functionally replace the damaged neurons, suggesting their potential as donor cells in developing replacement medicine for neurodegenerative diseases. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2019).

Original languageEnglish
Article number100346
JournalSTAR Protocols
Issue number1
Publication statusPublished - 19 Mar 2021


  • Cell differentiation
  • Cell isolation
  • Neuroscience
  • Stem cells

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