TY - JOUR
T1 - Oxidants and antioxidants affect the expression of glycodelin
AU - Ramachandran, Sumathi
AU - Rong, Rong
AU - Parthasarathy, Sampath
N1 - Funding Information:
This work was supported by grant 5POIHD35276 from the National Institute of Child and Human Development.
PY - 2003/4/1
Y1 - 2003/4/1
N2 - Glycodelin is a glycoprotein that has immunosuppressive activity. We have shown that K562 cells, hematopoitic progenitor cells, are capable of synthesizing glycodelin peptide (Gp) and, perhaps, contribute to Gp in tissues. In addition, several reproductive and nonreproductive tissues themselves are capable of synthesis of glycodelin. In this study, we report that lipid peroxides induce the synthesis of Gp. Antioxidants vitamin E and pyrrolidine dithiocarbamate (PDTC) and antioxidizing enzymes catalase and superoxide dismutase (SOD) effectively blocked phorbol myristate acetate- (PMA-) and lyso phosphatidic acid- (LPA-) induced synthesis of Gp. Dioctanoin (a mimic of diacylglycerol) activated Gp synthesis, and an inhibitor of protein kinase C (PKC) downregulated the response. Based on these observations, we postulate that oxidants by way of PKC might potentiate the angiogenic process.
AB - Glycodelin is a glycoprotein that has immunosuppressive activity. We have shown that K562 cells, hematopoitic progenitor cells, are capable of synthesizing glycodelin peptide (Gp) and, perhaps, contribute to Gp in tissues. In addition, several reproductive and nonreproductive tissues themselves are capable of synthesis of glycodelin. In this study, we report that lipid peroxides induce the synthesis of Gp. Antioxidants vitamin E and pyrrolidine dithiocarbamate (PDTC) and antioxidizing enzymes catalase and superoxide dismutase (SOD) effectively blocked phorbol myristate acetate- (PMA-) and lyso phosphatidic acid- (LPA-) induced synthesis of Gp. Dioctanoin (a mimic of diacylglycerol) activated Gp synthesis, and an inhibitor of protein kinase C (PKC) downregulated the response. Based on these observations, we postulate that oxidants by way of PKC might potentiate the angiogenic process.
KW - Angiogenesis
KW - Antioxidants
KW - Free radicals
KW - Glycodelin
KW - Oxidants
KW - Tumor promotion
UR - http://www.scopus.com/inward/record.url?scp=0037376866&partnerID=8YFLogxK
U2 - 10.1016/S0891-5849(02)01432-6
DO - 10.1016/S0891-5849(02)01432-6
M3 - Article
C2 - 12654469
AN - SCOPUS:0037376866
SN - 0891-5849
VL - 34
SP - 818
EP - 823
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
IS - 7
ER -