TY - GEN
T1 - Molecule studying on the diversity of cytoplasm fertility in chinese sugar beet germplasm resource
AU - Cheng, Dayou
AU - Cui, Jie
AU - Luo, Chengfei
AU - Liu, Qiaohong
AU - Shi, Shizhi
AU - Lu, Zhaoxin
AU - Li, Shilong
AU - Xu, Dechang
AU - Mikami, Tetuo
AU - Kobo, Tomohiko
PY - 2011
Y1 - 2011
N2 - To get and illustrate cytoplasm fertility polymorphism distribution among Sumfeng cultivars of sugar beet in china, some loci or region correlated with fertility in sugar beet mitochondrial genome and chloroplast genome were analyzed using molecular marker, the result will provide a strategy and foundation for selecting sugar beet male-sterile lines and O-type lines. The gene loci of minisatellite tandem repeat sequences (VNTRs) in the mitochondrial genomes of 73 sugar beet varieties(lines) were amplificated by PCR method, the reserve sequences between petG-psbE region in sugar beet chloroplast genomes were analyzed using PCR-RFLP method. All cultivars studied in this paper mainly center around three groups. 21 cultivars possess normal cytoplasm (1 model or 2 model or both). 14 cultivars (lines) only contain the Owen male-sterile cytoplasm. 25 cultivars(lines)contain both normall model cytoplasm and the Owen cytoplasm, so this 25 cultivars can be used as sugar beet resources for selecting improved O-type varieties (lines). K-5 cytoplasm is first found in sugar beet, it is essential to do more intensive study. S-2 male-sterile cytoplasm is first found in Chinese sugar beet cultivars, it can enrich male-sterile cytoplasm resources of Chinese sugar beet in a short time. Protein bolting analyzing showed that the proteins related with male-sterile only express in plants with the Owen cytoplasm, can't express in plants with 1 or 2 model cytoplasm. The cytoplasm types of Sumfeng varieties (lines) are more single. It is of vital importance to look for new types of cytoplasm to enrich sugar beet resource genetic background. Identifying cytoplasm fertility characters using molecular marker can grasp cytoplasm fertility of germplasm resources fast and exactly, and improve efficiency for breeding sugar beet male-sterile or O-type lines.
AB - To get and illustrate cytoplasm fertility polymorphism distribution among Sumfeng cultivars of sugar beet in china, some loci or region correlated with fertility in sugar beet mitochondrial genome and chloroplast genome were analyzed using molecular marker, the result will provide a strategy and foundation for selecting sugar beet male-sterile lines and O-type lines. The gene loci of minisatellite tandem repeat sequences (VNTRs) in the mitochondrial genomes of 73 sugar beet varieties(lines) were amplificated by PCR method, the reserve sequences between petG-psbE region in sugar beet chloroplast genomes were analyzed using PCR-RFLP method. All cultivars studied in this paper mainly center around three groups. 21 cultivars possess normal cytoplasm (1 model or 2 model or both). 14 cultivars (lines) only contain the Owen male-sterile cytoplasm. 25 cultivars(lines)contain both normall model cytoplasm and the Owen cytoplasm, so this 25 cultivars can be used as sugar beet resources for selecting improved O-type varieties (lines). K-5 cytoplasm is first found in sugar beet, it is essential to do more intensive study. S-2 male-sterile cytoplasm is first found in Chinese sugar beet cultivars, it can enrich male-sterile cytoplasm resources of Chinese sugar beet in a short time. Protein bolting analyzing showed that the proteins related with male-sterile only express in plants with the Owen cytoplasm, can't express in plants with 1 or 2 model cytoplasm. The cytoplasm types of Sumfeng varieties (lines) are more single. It is of vital importance to look for new types of cytoplasm to enrich sugar beet resource genetic background. Identifying cytoplasm fertility characters using molecular marker can grasp cytoplasm fertility of germplasm resources fast and exactly, and improve efficiency for breeding sugar beet male-sterile or O-type lines.
KW - Chloroplast genome
KW - Cytoplasm male-sterile
KW - Mitochondrial genome
KW - Sugar beet
UR - http://www.scopus.com/inward/record.url?scp=80052195111&partnerID=8YFLogxK
U2 - 10.1109/RSETE.2011.5966154
DO - 10.1109/RSETE.2011.5966154
M3 - Conference Proceeding
AN - SCOPUS:80052195111
SN - 9781424491711
T3 - 2011 International Conference on Remote Sensing, Environment and Transportation Engineering, RSETE 2011 - Proceedings
SP - 7681
EP - 7686
BT - 2011 International Conference on Remote Sensing, Environment and Transportation Engineering, RSETE 2011 - Proceedings
T2 - 2011 International Conference on Remote Sensing, Environment and Transportation Engineering, RSETE 2011
Y2 - 24 June 2011 through 26 June 2011
ER -