Long latency of evoked quantal transmitter release from somata of locus coeruleus neurons in rat pontine slices

H. P. Huang; S. R. Wang; W. Yao; C. Zhang; Y. Zhou; X. W. Chen; B. Zhang; W. Xiong; L. Y. Wang; L. H. Zheng; M. Landry; T. Hökfelt; Z. Q.D. Xu; Z. Zhou

doi:10.1073/pnas.0608897104

Long latency of evoked quantal transmitter release from somata of locus coeruleus neurons in rat pontine slices

H. P. Huang, S. R. Wang, W. Yao, C. Zhang, Y. Zhou, X. W. Chen, B. Zhang, W. Xiong, L. Y. Wang, L. H. Zheng, M. Landry, T. Hökfelt^*, Z. Q.D. Xu, Z. Zhou

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

60 Citations (Scopus)

Abstract

The locus coeruleus (LC) harbors a compact group of noradrenergic cell bodies projecting to virtually all parts of the central nervous system. By using combined measurements of amperometry and patch-clamp, quantal vesicle release of noradrenaline (NA) was detected as amperometric spikes, after depolarization of the LC neurons. After a pulse depolarization, the average latency of amperometric spikes was 1,870 ms, whereas the latency of glutamate-mediated excitatory postsynaptic currents was 1.6 ms. A substantial fraction of the depolarization-induced amperometric spikes originated from the somata. In contrast to glutamate-mediated excitatory postsynaptic currents, NA secretion was strongly modulated by the action potential frequency (0.5-50 Hz). Somatodendritic NA release from LC upon enhanced cell activity produced autoinhibition of firing and of NA release. We conclude that, in contrast to classic synaptic transmission, quantal NA release from LC somata is characterized by a number of distinct properties, including long latency and high sensitivity to action potential frequency.

Original language	English
Pages (from-to)	1401-1406
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	104
Issue number	4
DOIs	https://doi.org/10.1073/pnas.0608897104
Publication status	Published - 23 Jan 2007
Externally published	Yes

Keywords

Amperometry
Brain slice
Catecholamine
Patch-clamp
Somatic release

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10.1073/pnas.0608897104

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Huang, H. P., Wang, S. R., Yao, W., Zhang, C., Zhou, Y., Chen, X. W., Zhang, B., Xiong, W., Wang, L. Y., Zheng, L. H., Landry, M., Hökfelt, T., Xu, Z. Q. D., & Zhou, Z. (2007). Long latency of evoked quantal transmitter release from somata of locus coeruleus neurons in rat pontine slices. Proceedings of the National Academy of Sciences of the United States of America, 104(4), 1401-1406. https://doi.org/10.1073/pnas.0608897104

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title = "Long latency of evoked quantal transmitter release from somata of locus coeruleus neurons in rat pontine slices",

abstract = "The locus coeruleus (LC) harbors a compact group of noradrenergic cell bodies projecting to virtually all parts of the central nervous system. By using combined measurements of amperometry and patch-clamp, quantal vesicle release of noradrenaline (NA) was detected as amperometric spikes, after depolarization of the LC neurons. After a pulse depolarization, the average latency of amperometric spikes was 1,870 ms, whereas the latency of glutamate-mediated excitatory postsynaptic currents was 1.6 ms. A substantial fraction of the depolarization-induced amperometric spikes originated from the somata. In contrast to glutamate-mediated excitatory postsynaptic currents, NA secretion was strongly modulated by the action potential frequency (0.5-50 Hz). Somatodendritic NA release from LC upon enhanced cell activity produced autoinhibition of firing and of NA release. We conclude that, in contrast to classic synaptic transmission, quantal NA release from LC somata is characterized by a number of distinct properties, including long latency and high sensitivity to action potential frequency.",

keywords = "Amperometry, Brain slice, Catecholamine, Patch-clamp, Somatic release",

author = "Huang, {H. P.} and Wang, {S. R.} and W. Yao and C. Zhang and Y. Zhou and Chen, {X. W.} and B. Zhang and W. Xiong and Wang, {L. Y.} and Zheng, {L. H.} and M. Landry and T. H{\"o}kfelt and Xu, {Z. Q.D.} and Z. Zhou",

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doi = "10.1073/pnas.0608897104",

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Huang, HP, Wang, SR, Yao, W, Zhang, C, Zhou, Y, Chen, XW, Zhang, B, Xiong, W, Wang, LY, Zheng, LH, Landry, M, Hökfelt, T, Xu, ZQD & Zhou, Z 2007, 'Long latency of evoked quantal transmitter release from somata of locus coeruleus neurons in rat pontine slices', Proceedings of the National Academy of Sciences of the United States of America, vol. 104, no. 4, pp. 1401-1406. https://doi.org/10.1073/pnas.0608897104

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T1 - Long latency of evoked quantal transmitter release from somata of locus coeruleus neurons in rat pontine slices

AU - Huang, H. P.

AU - Wang, S. R.

AU - Yao, W.

AU - Zhang, C.

AU - Zhou, Y.

AU - Chen, X. W.

AU - Zhang, B.

AU - Xiong, W.

AU - Wang, L. Y.

AU - Zheng, L. H.

AU - Landry, M.

AU - Hökfelt, T.

AU - Xu, Z. Q.D.

AU - Zhou, Z.

PY - 2007/1/23

Y1 - 2007/1/23

N2 - The locus coeruleus (LC) harbors a compact group of noradrenergic cell bodies projecting to virtually all parts of the central nervous system. By using combined measurements of amperometry and patch-clamp, quantal vesicle release of noradrenaline (NA) was detected as amperometric spikes, after depolarization of the LC neurons. After a pulse depolarization, the average latency of amperometric spikes was 1,870 ms, whereas the latency of glutamate-mediated excitatory postsynaptic currents was 1.6 ms. A substantial fraction of the depolarization-induced amperometric spikes originated from the somata. In contrast to glutamate-mediated excitatory postsynaptic currents, NA secretion was strongly modulated by the action potential frequency (0.5-50 Hz). Somatodendritic NA release from LC upon enhanced cell activity produced autoinhibition of firing and of NA release. We conclude that, in contrast to classic synaptic transmission, quantal NA release from LC somata is characterized by a number of distinct properties, including long latency and high sensitivity to action potential frequency.

AB - The locus coeruleus (LC) harbors a compact group of noradrenergic cell bodies projecting to virtually all parts of the central nervous system. By using combined measurements of amperometry and patch-clamp, quantal vesicle release of noradrenaline (NA) was detected as amperometric spikes, after depolarization of the LC neurons. After a pulse depolarization, the average latency of amperometric spikes was 1,870 ms, whereas the latency of glutamate-mediated excitatory postsynaptic currents was 1.6 ms. A substantial fraction of the depolarization-induced amperometric spikes originated from the somata. In contrast to glutamate-mediated excitatory postsynaptic currents, NA secretion was strongly modulated by the action potential frequency (0.5-50 Hz). Somatodendritic NA release from LC upon enhanced cell activity produced autoinhibition of firing and of NA release. We conclude that, in contrast to classic synaptic transmission, quantal NA release from LC somata is characterized by a number of distinct properties, including long latency and high sensitivity to action potential frequency.

KW - Amperometry

KW - Brain slice

KW - Catecholamine

KW - Patch-clamp

KW - Somatic release

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JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 4

ER -

Long latency of evoked quantal transmitter release from somata of locus coeruleus neurons in rat pontine slices

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Keywords

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