Li, T., Kong, L., Li, X., Wu, S., Attri, K. S., Li, Y., Gong, W., Zhao, B., Li, L., Herring, L. E., Asara, J. M., Xu, L., Luo, X., Lei, Y. L., Ma, Q., Seveau, S., Gunn, J. S., Cheng, X., Singh, P. K., ... Wen, H. (2021). Listeria monocytogenes upregulates mitochondrial calcium signalling to inhibit LC3-associated phagocytosis as a survival strategy. Nature Microbiology, 6(3), 366-379. https://doi.org/10.1038/s41564-020-00843-2
Li, Tianliang ; Kong, Ligang ; Li, Xinghui et al. / Listeria monocytogenes upregulates mitochondrial calcium signalling to inhibit LC3-associated phagocytosis as a survival strategy. In: Nature Microbiology. 2021 ; Vol. 6, No. 3. pp. 366-379.
@article{ecd6cd20ddda472e99076cd835469dd7,
title = "Listeria monocytogenes upregulates mitochondrial calcium signalling to inhibit LC3-associated phagocytosis as a survival strategy",
abstract = "Mitochondria are believed to have originated ~2.5 billion years ago. As well as energy generation in cells, mitochondria have a role in defence against bacterial pathogens. Despite profound changes in mitochondrial morphology and functions following bacterial challenge, whether intracellular bacteria can hijack mitochondria to promote their survival remains elusive. We report that Listeria monocytogenes—an intracellular bacterial pathogen—suppresses LC3-associated phagocytosis (LAP) by modulation of mitochondrial Ca2+ (mtCa2+) signalling in order to survive inside cells. Invasion of macrophages by L. monocytogenes induced mtCa2+ uptake through the mtCa2+ uniporter (MCU), which in turn increased acetyl-coenzyme A (acetyl-CoA) production by pyruvate dehydrogenase. Acetylation of the LAP effector Rubicon with acetyl-CoA decreased LAP formation. Genetic ablation of MCU attenuated intracellular bacterial growth due to increased LAP formation. Our data show that modulation of mtCa2+ signalling can increase bacterial survival inside cells, and highlight the importance of mitochondrial metabolism in host–microbial interactions.",
author = "Tianliang Li and Ligang Kong and Xinghui Li and Sijin Wu and Attri, {Kuldeep S.} and Yan Li and Weipeng Gong and Bao Zhao and Lupeng Li and Herring, {Laura E.} and Asara, {John M.} and Lei Xu and Xiaobo Luo and Lei, {Yu L.} and Qin Ma and Stephanie Seveau and Gunn, {John S.} and Xiaolin Cheng and Singh, {Pankaj K.} and Green, {Douglas R.} and Haibo Wang and Haitao Wen",
note = "Publisher Copyright: {\textcopyright} 2021, The Author(s), under exclusive licence to Springer Nature Limited.",
year = "2021",
month = mar,
doi = "10.1038/s41564-020-00843-2",
language = "English",
volume = "6",
pages = "366--379",
journal = "Nature Microbiology",
issn = "2058-5276",
number = "3",
}
Li, T, Kong, L, Li, X, Wu, S, Attri, KS, Li, Y, Gong, W, Zhao, B, Li, L, Herring, LE, Asara, JM, Xu, L, Luo, X, Lei, YL, Ma, Q, Seveau, S, Gunn, JS, Cheng, X, Singh, PK, Green, DR, Wang, H & Wen, H 2021, 'Listeria monocytogenes upregulates mitochondrial calcium signalling to inhibit LC3-associated phagocytosis as a survival strategy', Nature Microbiology, vol. 6, no. 3, pp. 366-379. https://doi.org/10.1038/s41564-020-00843-2
Listeria monocytogenes upregulates mitochondrial calcium signalling to inhibit LC3-associated phagocytosis as a survival strategy. / Li, Tianliang; Kong, Ligang; Li, Xinghui et al.
In:
Nature Microbiology, Vol. 6, No. 3, 03.2021, p. 366-379.
Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - Listeria monocytogenes upregulates mitochondrial calcium signalling to inhibit LC3-associated phagocytosis as a survival strategy
AU - Li, Tianliang
AU - Kong, Ligang
AU - Li, Xinghui
AU - Wu, Sijin
AU - Attri, Kuldeep S.
AU - Li, Yan
AU - Gong, Weipeng
AU - Zhao, Bao
AU - Li, Lupeng
AU - Herring, Laura E.
AU - Asara, John M.
AU - Xu, Lei
AU - Luo, Xiaobo
AU - Lei, Yu L.
AU - Ma, Qin
AU - Seveau, Stephanie
AU - Gunn, John S.
AU - Cheng, Xiaolin
AU - Singh, Pankaj K.
AU - Green, Douglas R.
AU - Wang, Haibo
AU - Wen, Haitao
N1 - Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer Nature Limited.
PY - 2021/3
Y1 - 2021/3
N2 - Mitochondria are believed to have originated ~2.5 billion years ago. As well as energy generation in cells, mitochondria have a role in defence against bacterial pathogens. Despite profound changes in mitochondrial morphology and functions following bacterial challenge, whether intracellular bacteria can hijack mitochondria to promote their survival remains elusive. We report that Listeria monocytogenes—an intracellular bacterial pathogen—suppresses LC3-associated phagocytosis (LAP) by modulation of mitochondrial Ca2+ (mtCa2+) signalling in order to survive inside cells. Invasion of macrophages by L. monocytogenes induced mtCa2+ uptake through the mtCa2+ uniporter (MCU), which in turn increased acetyl-coenzyme A (acetyl-CoA) production by pyruvate dehydrogenase. Acetylation of the LAP effector Rubicon with acetyl-CoA decreased LAP formation. Genetic ablation of MCU attenuated intracellular bacterial growth due to increased LAP formation. Our data show that modulation of mtCa2+ signalling can increase bacterial survival inside cells, and highlight the importance of mitochondrial metabolism in host–microbial interactions.
AB - Mitochondria are believed to have originated ~2.5 billion years ago. As well as energy generation in cells, mitochondria have a role in defence against bacterial pathogens. Despite profound changes in mitochondrial morphology and functions following bacterial challenge, whether intracellular bacteria can hijack mitochondria to promote their survival remains elusive. We report that Listeria monocytogenes—an intracellular bacterial pathogen—suppresses LC3-associated phagocytosis (LAP) by modulation of mitochondrial Ca2+ (mtCa2+) signalling in order to survive inside cells. Invasion of macrophages by L. monocytogenes induced mtCa2+ uptake through the mtCa2+ uniporter (MCU), which in turn increased acetyl-coenzyme A (acetyl-CoA) production by pyruvate dehydrogenase. Acetylation of the LAP effector Rubicon with acetyl-CoA decreased LAP formation. Genetic ablation of MCU attenuated intracellular bacterial growth due to increased LAP formation. Our data show that modulation of mtCa2+ signalling can increase bacterial survival inside cells, and highlight the importance of mitochondrial metabolism in host–microbial interactions.
UR - http://www.scopus.com/inward/record.url?scp=85099343822&partnerID=8YFLogxK
U2 - 10.1038/s41564-020-00843-2
DO - 10.1038/s41564-020-00843-2
M3 - Article
C2 - 33462436
AN - SCOPUS:85099343822
SN - 2058-5276
VL - 6
SP - 366
EP - 379
JO - Nature Microbiology
JF - Nature Microbiology
IS - 3
ER -
Li T, Kong L, Li X, Wu S, Attri KS, Li Y et al. Listeria monocytogenes upregulates mitochondrial calcium signalling to inhibit LC3-associated phagocytosis as a survival strategy. Nature Microbiology. 2021 Mar;6(3):366-379. doi: 10.1038/s41564-020-00843-2
