Differential expression of TIM-3 in circulation and tumor microenvironment of colorectal cancer patients

Sarah Khalaf; Salman M. Toor; Khaled Murshed; Mohamed A. Kurer; Ayman A. Ahmed; Mohamed Abu Nada; Eyad Elkord

doi:10.1016/j.clim.2020.108429

Differential expression of TIM-3 in circulation and tumor microenvironment of colorectal cancer patients

Sarah Khalaf, Salman M. Toor, Khaled Murshed, Mohamed A. Kurer, Ayman A. Ahmed, Mohamed Abu Nada, Eyad Elkord^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

T-cell immunoglobulin and mucin-domain containing-3 (TIM-3) is an inhibitory immune checkpoint, which suppresses anti-tumor immune responses. TIM-3 expression on different immune cells in periphery and tumor microenvironment (TME) of colorectal cancer (CRC) patients has not been fully investigated. We found that TIM-3 was mainly expressed on monocytic myeloid cells (MMCs) and antigen-presenting cells (APCs) in circulation but was mainly expressed on T cells and APCs in the TME. Additionally, TIM-3⁻ T cells co-expressed higher levels of PD-1 than TIM-3⁺ T cells in normal tissue. In contrast, TIM-3⁺ T cells in the TME showed significantly higher PD-1 expression. Interestingly, there was a trend towards increased levels of TIM-3⁺ APCs with disease stages; however, levels of TIM-3⁺ T cells were decreased with disease stages in the TME. This study shows the differential expression of TIM-3 on different immune cells in circulation and TME of CRC patients, and their associations with disease stages.

Original language	English
Article number	108429
Journal	Clinical Immunology
Volume	215
DOIs	https://doi.org/10.1016/j.clim.2020.108429
Publication status	Published - Jun 2020
Externally published	Yes

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@article{702937f9ec4f427ba33fa12ec2486ba0,

title = "Differential expression of TIM-3 in circulation and tumor microenvironment of colorectal cancer patients",

abstract = "T-cell immunoglobulin and mucin-domain containing-3 (TIM-3) is an inhibitory immune checkpoint, which suppresses anti-tumor immune responses. TIM-3 expression on different immune cells in periphery and tumor microenvironment (TME) of colorectal cancer (CRC) patients has not been fully investigated. We found that TIM-3 was mainly expressed on monocytic myeloid cells (MMCs) and antigen-presenting cells (APCs) in circulation but was mainly expressed on T cells and APCs in the TME. Additionally, TIM-3− T cells co-expressed higher levels of PD-1 than TIM-3+ T cells in normal tissue. In contrast, TIM-3+ T cells in the TME showed significantly higher PD-1 expression. Interestingly, there was a trend towards increased levels of TIM-3+ APCs with disease stages; however, levels of TIM-3+ T cells were decreased with disease stages in the TME. This study shows the differential expression of TIM-3 on different immune cells in circulation and TME of CRC patients, and their associations with disease stages.",

author = "Sarah Khalaf and Toor, {Salman M.} and Khaled Murshed and Kurer, {Mohamed A.} and Ahmed, {Ayman A.} and {Abu Nada}, Mohamed and Eyad Elkord",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",

year = "2020",

month = jun,

doi = "10.1016/j.clim.2020.108429",

language = "English",

volume = "215",

journal = "Clinical Immunology",

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T1 - Differential expression of TIM-3 in circulation and tumor microenvironment of colorectal cancer patients

AU - Khalaf, Sarah

AU - Toor, Salman M.

AU - Murshed, Khaled

AU - Kurer, Mohamed A.

AU - Ahmed, Ayman A.

AU - Abu Nada, Mohamed

AU - Elkord, Eyad

PY - 2020/6

Y1 - 2020/6

N2 - T-cell immunoglobulin and mucin-domain containing-3 (TIM-3) is an inhibitory immune checkpoint, which suppresses anti-tumor immune responses. TIM-3 expression on different immune cells in periphery and tumor microenvironment (TME) of colorectal cancer (CRC) patients has not been fully investigated. We found that TIM-3 was mainly expressed on monocytic myeloid cells (MMCs) and antigen-presenting cells (APCs) in circulation but was mainly expressed on T cells and APCs in the TME. Additionally, TIM-3− T cells co-expressed higher levels of PD-1 than TIM-3+ T cells in normal tissue. In contrast, TIM-3+ T cells in the TME showed significantly higher PD-1 expression. Interestingly, there was a trend towards increased levels of TIM-3+ APCs with disease stages; however, levels of TIM-3+ T cells were decreased with disease stages in the TME. This study shows the differential expression of TIM-3 on different immune cells in circulation and TME of CRC patients, and their associations with disease stages.

AB - T-cell immunoglobulin and mucin-domain containing-3 (TIM-3) is an inhibitory immune checkpoint, which suppresses anti-tumor immune responses. TIM-3 expression on different immune cells in periphery and tumor microenvironment (TME) of colorectal cancer (CRC) patients has not been fully investigated. We found that TIM-3 was mainly expressed on monocytic myeloid cells (MMCs) and antigen-presenting cells (APCs) in circulation but was mainly expressed on T cells and APCs in the TME. Additionally, TIM-3− T cells co-expressed higher levels of PD-1 than TIM-3+ T cells in normal tissue. In contrast, TIM-3+ T cells in the TME showed significantly higher PD-1 expression. Interestingly, there was a trend towards increased levels of TIM-3+ APCs with disease stages; however, levels of TIM-3+ T cells were decreased with disease stages in the TME. This study shows the differential expression of TIM-3 on different immune cells in circulation and TME of CRC patients, and their associations with disease stages.

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SN - 1521-6616

VL - 215

JO - Clinical Immunology

JF - Clinical Immunology

M1 - 108429

ER -

Differential expression of TIM-3 in circulation and tumor microenvironment of colorectal cancer patients

Abstract

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