TY - GEN
T1 - Aptasensor Made of Carbon-Based Nanostructures for Detecting Spermine
AU - Tang, Howyn
AU - Pouri, Hossein
AU - Chen, Wei
AU - Lu, Chao
AU - Zhang, Jin
N1 - Publisher Copyright:
© 2023 IEEE.
PY - 2023
Y1 - 2023
N2 - Spermine, a type of polyamine, plays an important role in regulating ion channels, inhibits fat formation, and promotes skeletal development and intestinal maturation. Recently, spermine has been considered as a biomarker for diagnosis of pancreatic cancer. In this paper, an aptasensor made of carbon nanostructures has been developed to quickly and quantitatively detect spermine in aqueous media by using the Förster resonance energy transfer (FRET) quenching technique. Carbon quantum dots (CDs), acting as the FRET donor, have an average diameter of 5.0±2.5 nm and show the maximum emission intensity (Imax) at 510 nm when the excitation (λex) is at 400 nm. The spermine DNA aptamer has been bioconjugated onto the surface of CDs. The Imax of CDs conjugated with the aptamer is decreased significantly after interacting with graphene oxide nanosheets (GO), the FRET quencher, because of the short distance between CDs and GO through coupling with the DNA aptamer. Due to the strong affinity between the DNA aptamer and spermine, the fluorescence intensity of the CDs-based DNA sensor is restored in the presence of spermine. The spermine detection range, 0.2-250 nM, of this DNA sensor made of carbon-based nanostructures, has been analyzed. This study can provide a cost-effective and user-friendly sensing system to quickly detect spermine, a biomarker associated with pancreatic cancer.
AB - Spermine, a type of polyamine, plays an important role in regulating ion channels, inhibits fat formation, and promotes skeletal development and intestinal maturation. Recently, spermine has been considered as a biomarker for diagnosis of pancreatic cancer. In this paper, an aptasensor made of carbon nanostructures has been developed to quickly and quantitatively detect spermine in aqueous media by using the Förster resonance energy transfer (FRET) quenching technique. Carbon quantum dots (CDs), acting as the FRET donor, have an average diameter of 5.0±2.5 nm and show the maximum emission intensity (Imax) at 510 nm when the excitation (λex) is at 400 nm. The spermine DNA aptamer has been bioconjugated onto the surface of CDs. The Imax of CDs conjugated with the aptamer is decreased significantly after interacting with graphene oxide nanosheets (GO), the FRET quencher, because of the short distance between CDs and GO through coupling with the DNA aptamer. Due to the strong affinity between the DNA aptamer and spermine, the fluorescence intensity of the CDs-based DNA sensor is restored in the presence of spermine. The spermine detection range, 0.2-250 nM, of this DNA sensor made of carbon-based nanostructures, has been analyzed. This study can provide a cost-effective and user-friendly sensing system to quickly detect spermine, a biomarker associated with pancreatic cancer.
UR - http://www.scopus.com/inward/record.url?scp=85173590130&partnerID=8YFLogxK
U2 - 10.1109/NANO58406.2023.10231200
DO - 10.1109/NANO58406.2023.10231200
M3 - Conference Proceeding
AN - SCOPUS:85173590130
T3 - Proceedings of the IEEE Conference on Nanotechnology
SP - 772
EP - 776
BT - 2023 IEEE 23rd International Conference on Nanotechnology, NANO 2023
PB - IEEE Computer Society
T2 - 23rd IEEE International Conference on Nanotechnology, NANO 2023
Y2 - 2 July 2023 through 5 July 2023
ER -
