TY - JOUR
T1 - Triple-mode sensing platform for acetylcholinesterase activity monitoring and anti-Alzheimer's drug screening based on a highly stable Cu (I) compound
AU - Cai, Yanting
AU - Li, Yue
AU - Wang, Yuanyuan
AU - Xu, Yihan
AU - Chen, Tianyan
AU - Xue, Ruisong
AU - Liu, Yanmei
AU - Chen, Wei
AU - Yang, Xiurong
AU - Liu, Zhen
AU - Bao, Xingfu
AU - Huang, Zhenzhen
N1 - Publisher Copyright:
© 2024 Elsevier B.V.
PY - 2025/3/1
Y1 - 2025/3/1
N2 - Acetylcholinesterase (AChE) and AChE inhibitors play critical roles in the early diagnosis and treatment of Alzheimer's disease (AD). Herein, a fluorescence/colorimetry/smartphone triple-mode sensing platform was constructed for both AChE activity monitoring and AChE inhibitor screening by exploring a Cu (I) compound, Cu3I (SR)2 (R = CH2CH2NH2), as a fluorescent probe. In comparison of most other fluorescent probes, Cu3I (SR)2 presented exceptional stability against pH, temperature, UV irradiation, redox agents, and metal ions, as well as good recyclability due to its unique chemical structure. We further found the fluorescence emission of Cu3I (SR)2 could be quenched by MnO2 nanosheet (NS) via inner filter effect, and restored by thiocholine (TCh) generated from the hydrolysis of acetylthiocholine iodide (ATCh) in the catalysis of AChE. On this basis, a fluorescence “turn-on” assay was developed for monitoring AChE activity with a detection limit of 0.03 U/L and a detection range of 0.25–50 U/L. This method demonstrates great potential for real-time detection of AChE activity in biological samples and screening of AChE inhibitors obtained from herbal extracts as anti-AD agents. Additionally, Cu3I (SR)2/MnO2 NS sensing system also exhibited a color change from brown to colorless as the increasing AChE activity, which allowed the colorimetric and smartphone detection of AChE activity.
AB - Acetylcholinesterase (AChE) and AChE inhibitors play critical roles in the early diagnosis and treatment of Alzheimer's disease (AD). Herein, a fluorescence/colorimetry/smartphone triple-mode sensing platform was constructed for both AChE activity monitoring and AChE inhibitor screening by exploring a Cu (I) compound, Cu3I (SR)2 (R = CH2CH2NH2), as a fluorescent probe. In comparison of most other fluorescent probes, Cu3I (SR)2 presented exceptional stability against pH, temperature, UV irradiation, redox agents, and metal ions, as well as good recyclability due to its unique chemical structure. We further found the fluorescence emission of Cu3I (SR)2 could be quenched by MnO2 nanosheet (NS) via inner filter effect, and restored by thiocholine (TCh) generated from the hydrolysis of acetylthiocholine iodide (ATCh) in the catalysis of AChE. On this basis, a fluorescence “turn-on” assay was developed for monitoring AChE activity with a detection limit of 0.03 U/L and a detection range of 0.25–50 U/L. This method demonstrates great potential for real-time detection of AChE activity in biological samples and screening of AChE inhibitors obtained from herbal extracts as anti-AD agents. Additionally, Cu3I (SR)2/MnO2 NS sensing system also exhibited a color change from brown to colorless as the increasing AChE activity, which allowed the colorimetric and smartphone detection of AChE activity.
KW - Acetylcholinesterase detection
KW - Acetylcholinesterase inhibitor screening
KW - Colorimetry
KW - Fluorometry
KW - Smartphone detection
UR - http://www.scopus.com/inward/record.url?scp=85212534311&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2024.117078
DO - 10.1016/j.bios.2024.117078
M3 - Article
C2 - 39708491
AN - SCOPUS:85212534311
SN - 0956-5663
VL - 271
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
M1 - 117078
ER -