The influence of nanotexturing of poly(lactic-co-glycolic acid) films upon human ovarian cancer cell attachment

Gökcen Yaşayan; Xuan Xue; Pamela Collier; Philip Clarke; Morgan R. Alexander; Maria Marlow

doi:10.1088/0957-4484/27/25/255102

The influence of nanotexturing of poly(lactic-co-glycolic acid) films upon human ovarian cancer cell attachment

Gökcen Yaşayan, Xuan Xue, Pamela Collier, Philip Clarke, Morgan R. Alexander, Maria Marlow

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

In this study, we have produced nanotextured poly(lactic-co-glycolic acid) (PLGA) films by using polystyrene (PS) particles as a template to make a polydimethylsiloxane mould against which PLGA is solvent cast. Biocompatible, biodegradable and nanotextured PLGA films were prepared with PS particles of diameter of 57, 99, 210, and 280 nm that produced domes of the same dimension in the PLGA surface. The effect of the particulate monolayer templating method was investigated to enable preparation of the films with uniformly ordered surface nanodomes. Cell attachment of a human ovarian cancer cell line (OVCAR3) alone and co-cultured with mesenchymal stem cells (MSCs) was evaluated on flat and topographically nano-patterned surfaces. Cell numbers were observed to increase on the nanotextured surfaces compared to non-textured surfaces both with OVCAR3 cultures and OVCAR3-MSC co-cultures at 24 and 48 h time points.

Original language	English
Article number	255102
Journal	Nanotechnology
Volume	27
Issue number	25
DOIs	https://doi.org/10.1088/0957-4484/27/25/255102
Publication status	Published - 17 May 2016
Externally published	Yes

Keywords

Nanotexturing
films
human ovarian cancer cell
poly(lactic-co-glycolic acid)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1088/0957-4484/27/25/255102

Cite this

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title = "The influence of nanotexturing of poly(lactic-co-glycolic acid) films upon human ovarian cancer cell attachment",

abstract = "In this study, we have produced nanotextured poly(lactic-co-glycolic acid) (PLGA) films by using polystyrene (PS) particles as a template to make a polydimethylsiloxane mould against which PLGA is solvent cast. Biocompatible, biodegradable and nanotextured PLGA films were prepared with PS particles of diameter of 57, 99, 210, and 280 nm that produced domes of the same dimension in the PLGA surface. The effect of the particulate monolayer templating method was investigated to enable preparation of the films with uniformly ordered surface nanodomes. Cell attachment of a human ovarian cancer cell line (OVCAR3) alone and co-cultured with mesenchymal stem cells (MSCs) was evaluated on flat and topographically nano-patterned surfaces. Cell numbers were observed to increase on the nanotextured surfaces compared to non-textured surfaces both with OVCAR3 cultures and OVCAR3-MSC co-cultures at 24 and 48 h time points.",

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AU - Yaşayan, Gökcen

AU - Xue, Xuan

AU - Collier, Pamela

AU - Clarke, Philip

AU - Alexander, Morgan R.

AU - Marlow, Maria

PY - 2016/5/17

Y1 - 2016/5/17

N2 - In this study, we have produced nanotextured poly(lactic-co-glycolic acid) (PLGA) films by using polystyrene (PS) particles as a template to make a polydimethylsiloxane mould against which PLGA is solvent cast. Biocompatible, biodegradable and nanotextured PLGA films were prepared with PS particles of diameter of 57, 99, 210, and 280 nm that produced domes of the same dimension in the PLGA surface. The effect of the particulate monolayer templating method was investigated to enable preparation of the films with uniformly ordered surface nanodomes. Cell attachment of a human ovarian cancer cell line (OVCAR3) alone and co-cultured with mesenchymal stem cells (MSCs) was evaluated on flat and topographically nano-patterned surfaces. Cell numbers were observed to increase on the nanotextured surfaces compared to non-textured surfaces both with OVCAR3 cultures and OVCAR3-MSC co-cultures at 24 and 48 h time points.

AB - In this study, we have produced nanotextured poly(lactic-co-glycolic acid) (PLGA) films by using polystyrene (PS) particles as a template to make a polydimethylsiloxane mould against which PLGA is solvent cast. Biocompatible, biodegradable and nanotextured PLGA films were prepared with PS particles of diameter of 57, 99, 210, and 280 nm that produced domes of the same dimension in the PLGA surface. The effect of the particulate monolayer templating method was investigated to enable preparation of the films with uniformly ordered surface nanodomes. Cell attachment of a human ovarian cancer cell line (OVCAR3) alone and co-cultured with mesenchymal stem cells (MSCs) was evaluated on flat and topographically nano-patterned surfaces. Cell numbers were observed to increase on the nanotextured surfaces compared to non-textured surfaces both with OVCAR3 cultures and OVCAR3-MSC co-cultures at 24 and 48 h time points.

KW - Nanotexturing

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KW - human ovarian cancer cell

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The influence of nanotexturing of poly(lactic-co-glycolic acid) films upon human ovarian cancer cell attachment

Abstract

Keywords

UN SDGs

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