The exploration of quantum dot-molecular beacon based MoS2 fluorescence probing for myeloma-related Mirnas detection

Jing Jing Wang; Ying Liu; Zhou Ding; Le Zhang; Caiqin Han; Changchun Yan; Eric Amador; Liqin Yuan; Ying Wu; Chunyuan Song; Wei Chen

doi:10.1016/j.bioactmat.2021.12.036

The exploration of quantum dot-molecular beacon based MoS₂ fluorescence probing for myeloma-related Mirnas detection

Jing Jing Wang, Ying Liu^*, Zhou Ding, Le Zhang, Caiqin Han, Changchun Yan, Eric Amador, Liqin Yuan, Ying Wu, Chunyuan Song, Wei Chen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Highly sensitive and reliable detection of multiple myeloma remains a major challenge in liquid biopsy. Herein, for the first time, quantum dot-molecular beacon (QD-MB) functionalized MoS₂ (QD-MB @MoS₂) fluorescent probes were designed for the dual detection of multiple myeloma (MM)-related miRNA-155 and miRNA-150. The results indicate that the two probes can effectively detect miRNA-155 and miRNA-150 simultaneously with satisfactory recovery rates, and the limit of detections (LODs) of miRNA-155 and miRNA-150 in human serum are low to 7.19 fM and 5.84 fM, respectively. These results indicate that our method is the most sensitive detection so far reported and that the designed fluorescent probes with signal amplification strategies can achieve highly sensitive detection of MM-related miRNAs for MM diagnosis.

Original language	English
Pages (from-to)	360-368
Number of pages	9
Journal	Bioactive Materials
Volume	17
DOIs	https://doi.org/10.1016/j.bioactmat.2021.12.036
Publication status	Published - Nov 2022
Externally published	Yes

Keywords

Duplex-specific nuclease (DSN)
miRNA-150
miRNA-155
Molecular beacon
MoS
Multiple myeloma
Quantum dot

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10.1016/j.bioactmat.2021.12.036

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@article{3e8432282afa460699eac663afff3617,

title = "The exploration of quantum dot-molecular beacon based MoS2 fluorescence probing for myeloma-related Mirnas detection",

abstract = "Highly sensitive and reliable detection of multiple myeloma remains a major challenge in liquid biopsy. Herein, for the first time, quantum dot-molecular beacon (QD-MB) functionalized MoS2 (QD-MB @MoS2) fluorescent probes were designed for the dual detection of multiple myeloma (MM)-related miRNA-155 and miRNA-150. The results indicate that the two probes can effectively detect miRNA-155 and miRNA-150 simultaneously with satisfactory recovery rates, and the limit of detections (LODs) of miRNA-155 and miRNA-150 in human serum are low to 7.19 fM and 5.84 fM, respectively. These results indicate that our method is the most sensitive detection so far reported and that the designed fluorescent probes with signal amplification strategies can achieve highly sensitive detection of MM-related miRNAs for MM diagnosis.",

keywords = "Duplex-specific nuclease (DSN), miRNA-150, miRNA-155, Molecular beacon, MoS, Multiple myeloma, Quantum dot",

author = "Wang, {Jing Jing} and Ying Liu and Zhou Ding and Le Zhang and Caiqin Han and Changchun Yan and Eric Amador and Liqin Yuan and Ying Wu and Chunyuan Song and Wei Chen",

note = "Publisher Copyright: {\textcopyright} 2022 The Authors",

year = "2022",

month = nov,

doi = "10.1016/j.bioactmat.2021.12.036",

language = "English",

volume = "17",

pages = "360--368",

journal = "Bioactive Materials",

issn = "2452-199X",

}

TY - JOUR

T1 - The exploration of quantum dot-molecular beacon based MoS2 fluorescence probing for myeloma-related Mirnas detection

AU - Wang, Jing Jing

AU - Liu, Ying

AU - Ding, Zhou

AU - Zhang, Le

AU - Han, Caiqin

AU - Yan, Changchun

AU - Amador, Eric

AU - Yuan, Liqin

AU - Wu, Ying

AU - Song, Chunyuan

AU - Chen, Wei

PY - 2022/11

Y1 - 2022/11

N2 - Highly sensitive and reliable detection of multiple myeloma remains a major challenge in liquid biopsy. Herein, for the first time, quantum dot-molecular beacon (QD-MB) functionalized MoS2 (QD-MB @MoS2) fluorescent probes were designed for the dual detection of multiple myeloma (MM)-related miRNA-155 and miRNA-150. The results indicate that the two probes can effectively detect miRNA-155 and miRNA-150 simultaneously with satisfactory recovery rates, and the limit of detections (LODs) of miRNA-155 and miRNA-150 in human serum are low to 7.19 fM and 5.84 fM, respectively. These results indicate that our method is the most sensitive detection so far reported and that the designed fluorescent probes with signal amplification strategies can achieve highly sensitive detection of MM-related miRNAs for MM diagnosis.

AB - Highly sensitive and reliable detection of multiple myeloma remains a major challenge in liquid biopsy. Herein, for the first time, quantum dot-molecular beacon (QD-MB) functionalized MoS2 (QD-MB @MoS2) fluorescent probes were designed for the dual detection of multiple myeloma (MM)-related miRNA-155 and miRNA-150. The results indicate that the two probes can effectively detect miRNA-155 and miRNA-150 simultaneously with satisfactory recovery rates, and the limit of detections (LODs) of miRNA-155 and miRNA-150 in human serum are low to 7.19 fM and 5.84 fM, respectively. These results indicate that our method is the most sensitive detection so far reported and that the designed fluorescent probes with signal amplification strategies can achieve highly sensitive detection of MM-related miRNAs for MM diagnosis.

KW - Duplex-specific nuclease (DSN)

KW - miRNA-150

KW - miRNA-155

KW - Molecular beacon

KW - MoS

KW - Multiple myeloma

KW - Quantum dot

UR - http://www.scopus.com/inward/record.url?scp=85122509447&partnerID=8YFLogxK

U2 - 10.1016/j.bioactmat.2021.12.036

DO - 10.1016/j.bioactmat.2021.12.036

M3 - Article

AN - SCOPUS:85122509447

SN - 2452-199X

VL - 17

SP - 360

EP - 368

JO - Bioactive Materials

JF - Bioactive Materials

ER -

The exploration of quantum dot-molecular beacon based MoS₂ fluorescence probing for myeloma-related Mirnas detection

Abstract

Keywords

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