Abstract
Nanoscience offers the potential for great
advances in medical technology and therapies in the form
of nanomedicine. As such, developing controllable, predictable, and effective, nanoparticle-based therapeutic
systems remains a significant challenge. Many polymerbased nanoparticle systems have been reported to date, but
few harness materials with accepted biocompatibility.
Phosphorylcholine (PC) based biomimetic materials have a
long history of successful translation into effective commercial medical technologies. This study investigated the
synthesis, characterisation, nanoprecipitation, and in vitro
cellular uptake kinetics of PC-based polymeric nanoparticle micelles (PNM) formed by the biocompatible and pH
responsive block copolymer poly(2-methacryloyloxyethyl
phosphorylcholine)-b-poly(2-(diisopropylamino)ethyl
methacrylate) (MPC-DPA). Atom transfer radical polymerisation (ATRP), and gel permeation chromatography
(GPC) were used to synthesise and characterise the welldefined MPC100-DPA100 polymer, revealing organic GPC,
using evaporative light scatter detection, to be more
accurate than aqueous GPC for this application. Subsequent nanoprecipitation investigations utilising photon
correlation spectroscopy (PCS) revealed PNM size
increased with polymer concentration, and conferred Cryostability. PNM diameters ranged from circa 64–69 nm, and
increased upon hydrophobic compound loading, circa
65–71 nm, with loading efficiencies of circa 60 %
achieved, whilst remaining monodisperse. In vitro studies
demonstrated that the PNM were of low cellular toxicity,
with colony formation and MTT assays, utilising V79 and
3T3 cells, yielding comparable results. Investigation of the
in vitro cellular uptake kinetics revealed rapid, 1 h, cellular
uptake of MPC100-DPA100 PNM delivered fluorescent
probes, with fluorescence persistence for 48 h. This paper
presents the first report of these novel findings, which
highlight the potential of the system for nanomedicine
application development
advances in medical technology and therapies in the form
of nanomedicine. As such, developing controllable, predictable, and effective, nanoparticle-based therapeutic
systems remains a significant challenge. Many polymerbased nanoparticle systems have been reported to date, but
few harness materials with accepted biocompatibility.
Phosphorylcholine (PC) based biomimetic materials have a
long history of successful translation into effective commercial medical technologies. This study investigated the
synthesis, characterisation, nanoprecipitation, and in vitro
cellular uptake kinetics of PC-based polymeric nanoparticle micelles (PNM) formed by the biocompatible and pH
responsive block copolymer poly(2-methacryloyloxyethyl
phosphorylcholine)-b-poly(2-(diisopropylamino)ethyl
methacrylate) (MPC-DPA). Atom transfer radical polymerisation (ATRP), and gel permeation chromatography
(GPC) were used to synthesise and characterise the welldefined MPC100-DPA100 polymer, revealing organic GPC,
using evaporative light scatter detection, to be more
accurate than aqueous GPC for this application. Subsequent nanoprecipitation investigations utilising photon
correlation spectroscopy (PCS) revealed PNM size
increased with polymer concentration, and conferred Cryostability. PNM diameters ranged from circa 64–69 nm, and
increased upon hydrophobic compound loading, circa
65–71 nm, with loading efficiencies of circa 60 %
achieved, whilst remaining monodisperse. In vitro studies
demonstrated that the PNM were of low cellular toxicity,
with colony formation and MTT assays, utilising V79 and
3T3 cells, yielding comparable results. Investigation of the
in vitro cellular uptake kinetics revealed rapid, 1 h, cellular
uptake of MPC100-DPA100 PNM delivered fluorescent
probes, with fluorescence persistence for 48 h. This paper
presents the first report of these novel findings, which
highlight the potential of the system for nanomedicine
application development
| Original language | English |
|---|---|
| Pages (from-to) | 1073-1094 |
| Number of pages | 22 |
| Journal | Applied Nanoscience |
| DOIs | |
| Publication status | Published - 2015 |