TY - JOUR
T1 - Studies on immunological and degranulation properties of a galectin-1 purified from goat (Capra hircus) heart
AU - Ashraf, Ghulam Md
AU - Mahmoud, Maged Mostafa
AU - Tabrez, Shams
N1 - Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/8
Y1 - 2018/8
N2 - Galectins are mammalian lectins characterized by affinity for β-galactosides and the presence of a conserved carbohydrate recognition domain (CRD). Galectins play crucial role in the causation and progression of deadly human diseases like cancer, neurodegenerative disorders and cardiovascular disorders. Available literature reports relevant roles of galectins in innate as well as adaptive immune responses, along with the modulation of acute inflammatory response. In the current study, we purified the goat heart galectin-1 (GHG-1) and carried out its extensive immunological studies. Immunodiffusion studies revealed that anti-GHG-1 antibodies recognize the GHG-1 more readily as compared to the other galectins, suggesting its preferred utilization in various recognition studies. Antigenic cross-reactivity between galectins isolated from different tissues and species suggest their evolutionary preserved fundamental biological roles. A gradual increase in the lysozyme release was evident when the neutrophils were treated with various neutrophil activating agents. The findings of the present study confirm the increase in lysozyme production under the presence of various neutrophil activators, and thus add new information on GHG-1 induced degranulation.
AB - Galectins are mammalian lectins characterized by affinity for β-galactosides and the presence of a conserved carbohydrate recognition domain (CRD). Galectins play crucial role in the causation and progression of deadly human diseases like cancer, neurodegenerative disorders and cardiovascular disorders. Available literature reports relevant roles of galectins in innate as well as adaptive immune responses, along with the modulation of acute inflammatory response. In the current study, we purified the goat heart galectin-1 (GHG-1) and carried out its extensive immunological studies. Immunodiffusion studies revealed that anti-GHG-1 antibodies recognize the GHG-1 more readily as compared to the other galectins, suggesting its preferred utilization in various recognition studies. Antigenic cross-reactivity between galectins isolated from different tissues and species suggest their evolutionary preserved fundamental biological roles. A gradual increase in the lysozyme release was evident when the neutrophils were treated with various neutrophil activating agents. The findings of the present study confirm the increase in lysozyme production under the presence of various neutrophil activators, and thus add new information on GHG-1 induced degranulation.
KW - Competition inhibition
KW - Degranulation
KW - Direct binding ELISA
KW - Dot blot assay
KW - Double immunodiffusion
KW - Goat heart galectin-1
KW - Lysozyme release
UR - http://www.scopus.com/inward/record.url?scp=85046681790&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2018.04.136
DO - 10.1016/j.ijbiomac.2018.04.136
M3 - Article
C2 - 29704605
AN - SCOPUS:85046681790
SN - 0141-8130
VL - 115
SP - 1183
EP - 1188
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -
