TY - JOUR
T1 - Sensitive and selective electrochemical biosensor based on ELP-OPH/BSA/TiO2NFs/AuNPs for determination of organophosphate pesticides with p-nitrophenyl substituent
AU - Bao, Jing
AU - Hou, Changjun
AU - Huo, Danqun
AU - Dong, Qiuchen
AU - Ma, Xiaoyu
AU - Sun, Xiangcheng
AU - Yang, Mei
AU - Galil, Khaled Hussein Abd El
AU - Chen, Wilfred
AU - Lei, Yu
N1 - Publisher Copyright:
© 2016 The Electrochemical Society. All rights reserved.
PY - 2017
Y1 - 2017
N2 - We report herein a new electrochemical bisosensor based on elastin-like polypeptide-organophosphate hydrolase/bovine serum albumin/titanium oxide nanofibers/gold nanoparticles (ELP-OPH/BSA/TiO2NFs/AuNPs) for highly sensitive and selective, rapid, one-step determination of organophosphate pesticides (OPs) with p-nitrophenyl substituent. ELP-OPH was purified from genetically engineered Escherichia coli (E. coli) taking the advantage of thermal-triggered phase-transition of ELP. The surface morphology and crystal structure of the as-prepared ELP-OPH/BSA/TiO2NFs/AuNPs nanocomposites were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray Diffraction (XRD), respectively. The electrochemical properties and catalytic behavior of the as-prepared electrodes for the determination of methyl parathion were systematically investigated using cyclic voltammetry (CV), differential pulse voltammetry (DPV) and amperometry (i–t). During OPs detection, OP compounds were selectively adsorbed to the TiO2NFs surface due to the strong affinity of its phosphoric group with TiO2NFs, while AuNPs were employed to provide a stable interface for promoting electron transfer between biomolecules and electrodes, thus enhanced sensing performance was accomplished. Under the optimized operating conditions, the as-prepared biosensor can detect methyl parathion with a wide dynamic ranges (up to 116.4 μM), a good sensitivity of 734 μA cm−2 mM−1 and the limit of detection (S/N = 3) as low as 29 nM. Its further application for determination of methyl parathion spiked into lake water samples was also demonstrated with an acceptable stability. All these features indicate that the as-developed ELP-OPH/BSA/TiO2NFs/AuNPs/GCE holds great promise in rapid, sensitive, specific, and reliable detection of organophosphates pesticides.
AB - We report herein a new electrochemical bisosensor based on elastin-like polypeptide-organophosphate hydrolase/bovine serum albumin/titanium oxide nanofibers/gold nanoparticles (ELP-OPH/BSA/TiO2NFs/AuNPs) for highly sensitive and selective, rapid, one-step determination of organophosphate pesticides (OPs) with p-nitrophenyl substituent. ELP-OPH was purified from genetically engineered Escherichia coli (E. coli) taking the advantage of thermal-triggered phase-transition of ELP. The surface morphology and crystal structure of the as-prepared ELP-OPH/BSA/TiO2NFs/AuNPs nanocomposites were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray Diffraction (XRD), respectively. The electrochemical properties and catalytic behavior of the as-prepared electrodes for the determination of methyl parathion were systematically investigated using cyclic voltammetry (CV), differential pulse voltammetry (DPV) and amperometry (i–t). During OPs detection, OP compounds were selectively adsorbed to the TiO2NFs surface due to the strong affinity of its phosphoric group with TiO2NFs, while AuNPs were employed to provide a stable interface for promoting electron transfer between biomolecules and electrodes, thus enhanced sensing performance was accomplished. Under the optimized operating conditions, the as-prepared biosensor can detect methyl parathion with a wide dynamic ranges (up to 116.4 μM), a good sensitivity of 734 μA cm−2 mM−1 and the limit of detection (S/N = 3) as low as 29 nM. Its further application for determination of methyl parathion spiked into lake water samples was also demonstrated with an acceptable stability. All these features indicate that the as-developed ELP-OPH/BSA/TiO2NFs/AuNPs/GCE holds great promise in rapid, sensitive, specific, and reliable detection of organophosphates pesticides.
UR - http://www.scopus.com/inward/record.url?scp=85037993128&partnerID=8YFLogxK
U2 - 10.1149/2.0311702jes
DO - 10.1149/2.0311702jes
M3 - Article
AN - SCOPUS:85037993128
SN - 0013-4651
VL - 164
SP - G17-G22
JO - Journal of the Electrochemical Society
JF - Journal of the Electrochemical Society
IS - 2
ER -