Selenium accumulation, antioxidant enzyme levels, and amino acids composition in ChineseMitten Crab (Eriocheir sinensis) fed selenium-biofortified corn

Linxi Yuan; Ru Zhang; Xuzhou Ma; Ling Yang; Qing Zheng; Dong Chen; Miao Li; Ting Fan; Yongxian Liu; Liping Pan; Xuebin Yin

doi:10.3390/nu10030318

Selenium accumulation, antioxidant enzyme levels, and amino acids composition in ChineseMitten Crab (Eriocheir sinensis) fed selenium-biofortified corn

Linxi Yuan, Ru Zhang, Xuzhou Ma, Ling Yang, Qing Zheng, Dong Chen, Miao Li, Ting Fan, Yongxian Liu, Liping Pan, Xuebin Yin^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The effects of selenium (Se)-biofortified corn on the total Se contents, the antioxidant enzyme levels, and the amino acids composition in Chinese mitten crab (Eriocheir sinensis) during the stage of the fifth shelling to maturity were investigated in the present study. The culture density of crabs was 600 per 667 m², and they were continuously fed 120.4 mg Se from Se-biofortified corn per 667m² every two days for 90 days. The results showed that the total muscle Se levels in the crabs were significantly increased (p < 0.05). Activities of hemolymph supernatant enzymes including alkaline phosphatase (AKP), lysozyme (LZM), glutathione peroxidase (GPx), and superoxide dismutase (SOD) were also enhanced (p < 0.05). The protein and crude fat levels at maturity were higher than those at the fourth molt. The levels of total essential amino acids (ΣEAAs) and total delicious amino acids (ΣDAAs) were significantly increased (p < 0.05). We demonstrate that the feeding of Se-biofortified corn could significantly improve total muscle Se concentrations and hemolymph supernatant antioxidant enzyme activities in Chinese mitten crab, and slow down the rapid decline of ΣEAAs and ΣDAAs at maturity, thus improving the nutritional quality of Chinese mitten crab.

Original language	English
Article number	318
Journal	Nutrients
Volume	10
Issue number	3
DOIs	https://doi.org/10.3390/nu10030318
Publication status	Published - 7 Mar 2018
Externally published	Yes

Keywords

Amino acids content
Antioxidant levels
Chinese mitten crab
Hemolymph supernatant
Muscle se level
Se-biofortified corn

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10.3390/nu10030318

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title = "Selenium accumulation, antioxidant enzyme levels, and amino acids composition in ChineseMitten Crab (Eriocheir sinensis) fed selenium-biofortified corn",

abstract = "The effects of selenium (Se)-biofortified corn on the total Se contents, the antioxidant enzyme levels, and the amino acids composition in Chinese mitten crab (Eriocheir sinensis) during the stage of the fifth shelling to maturity were investigated in the present study. The culture density of crabs was 600 per 667 m2, and they were continuously fed 120.4 mg Se from Se-biofortified corn per 667m2 every two days for 90 days. The results showed that the total muscle Se levels in the crabs were significantly increased (p < 0.05). Activities of hemolymph supernatant enzymes including alkaline phosphatase (AKP), lysozyme (LZM), glutathione peroxidase (GPx), and superoxide dismutase (SOD) were also enhanced (p < 0.05). The protein and crude fat levels at maturity were higher than those at the fourth molt. The levels of total essential amino acids (ΣEAAs) and total delicious amino acids (ΣDAAs) were significantly increased (p < 0.05). We demonstrate that the feeding of Se-biofortified corn could significantly improve total muscle Se concentrations and hemolymph supernatant antioxidant enzyme activities in Chinese mitten crab, and slow down the rapid decline of ΣEAAs and ΣDAAs at maturity, thus improving the nutritional quality of Chinese mitten crab.",

keywords = "Amino acids content, Antioxidant levels, Chinese mitten crab, Hemolymph supernatant, Muscle se level, Se-biofortified corn",

author = "Linxi Yuan and Ru Zhang and Xuzhou Ma and Ling Yang and Qing Zheng and Dong Chen and Miao Li and Ting Fan and Yongxian Liu and Liping Pan and Xuebin Yin",

note = "Publisher Copyright: {\textcopyright} 2018 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2018",

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doi = "10.3390/nu10030318",

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T1 - Selenium accumulation, antioxidant enzyme levels, and amino acids composition in ChineseMitten Crab (Eriocheir sinensis) fed selenium-biofortified corn

AU - Yuan, Linxi

AU - Zhang, Ru

AU - Ma, Xuzhou

AU - Yang, Ling

AU - Zheng, Qing

AU - Chen, Dong

AU - Li, Miao

AU - Fan, Ting

AU - Liu, Yongxian

AU - Pan, Liping

AU - Yin, Xuebin

PY - 2018/3/7

Y1 - 2018/3/7

N2 - The effects of selenium (Se)-biofortified corn on the total Se contents, the antioxidant enzyme levels, and the amino acids composition in Chinese mitten crab (Eriocheir sinensis) during the stage of the fifth shelling to maturity were investigated in the present study. The culture density of crabs was 600 per 667 m2, and they were continuously fed 120.4 mg Se from Se-biofortified corn per 667m2 every two days for 90 days. The results showed that the total muscle Se levels in the crabs were significantly increased (p < 0.05). Activities of hemolymph supernatant enzymes including alkaline phosphatase (AKP), lysozyme (LZM), glutathione peroxidase (GPx), and superoxide dismutase (SOD) were also enhanced (p < 0.05). The protein and crude fat levels at maturity were higher than those at the fourth molt. The levels of total essential amino acids (ΣEAAs) and total delicious amino acids (ΣDAAs) were significantly increased (p < 0.05). We demonstrate that the feeding of Se-biofortified corn could significantly improve total muscle Se concentrations and hemolymph supernatant antioxidant enzyme activities in Chinese mitten crab, and slow down the rapid decline of ΣEAAs and ΣDAAs at maturity, thus improving the nutritional quality of Chinese mitten crab.

AB - The effects of selenium (Se)-biofortified corn on the total Se contents, the antioxidant enzyme levels, and the amino acids composition in Chinese mitten crab (Eriocheir sinensis) during the stage of the fifth shelling to maturity were investigated in the present study. The culture density of crabs was 600 per 667 m2, and they were continuously fed 120.4 mg Se from Se-biofortified corn per 667m2 every two days for 90 days. The results showed that the total muscle Se levels in the crabs were significantly increased (p < 0.05). Activities of hemolymph supernatant enzymes including alkaline phosphatase (AKP), lysozyme (LZM), glutathione peroxidase (GPx), and superoxide dismutase (SOD) were also enhanced (p < 0.05). The protein and crude fat levels at maturity were higher than those at the fourth molt. The levels of total essential amino acids (ΣEAAs) and total delicious amino acids (ΣDAAs) were significantly increased (p < 0.05). We demonstrate that the feeding of Se-biofortified corn could significantly improve total muscle Se concentrations and hemolymph supernatant antioxidant enzyme activities in Chinese mitten crab, and slow down the rapid decline of ΣEAAs and ΣDAAs at maturity, thus improving the nutritional quality of Chinese mitten crab.

KW - Amino acids content

KW - Antioxidant levels

KW - Chinese mitten crab

KW - Hemolymph supernatant

KW - Muscle se level

KW - Se-biofortified corn

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ER -

Selenium accumulation, antioxidant enzyme levels, and amino acids composition in ChineseMitten Crab (Eriocheir sinensis) fed selenium-biofortified corn

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