Scanning mutagenesis of transmembrane domain 3 of the M1 muscarinic acetylcholine receptor

Edward C. Hulme*, Zhi Liang Lu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Scanning mutagenesis of transmembrane domain 3 of the M1 muscarinic acetylcholine receptor has revealed a highly-differentiated α-helical structure. Lipid-facing residues are distinguished from a patch of residues which selectively stabilise the ground state of the receptor, and from a band of amino acids extending the full length of the helix, which contribute to the active agonist-receptor-G protein complex. The most important residues are strongly conserved in the GPCR superfamily.

Original languageEnglish
Pages (from-to)269-274
Number of pages6
JournalJournal of Physiology Paris
Volume92
Issue number3-4
DOIs
Publication statusPublished - 1998
Externally publishedYes

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