TY - JOUR
T1 - [Real-time measurement of noradrenaline release in central nervous system].
AU - Huang, Wei
AU - Huang, Hong Ping
AU - Mu, Y.
AU - Zhang, Lei
AU - Jin, M.
AU - Lv, Jing
AU - Gu, Jing Li
AU - Xiu, Yun
AU - Zhang, B.
AU - Guo, Ning
AU - Liu, Tao
AU - Sun, Lei
AU - Song, Mei Ying
AU - Zhang, Claire Xi
AU - Ruan, Huai Zhen
AU - Zhou, Zhuan
PY - 2007/12/25
Y1 - 2007/12/25
N2 - In order to investigate the central nervous mechanism and the diseases involved in catecholamine transmitter secretion, the dynamics of catecholamine release is studied in single cell, brain slice or in vivo. Noradrenaline is an important neurotransmitter and modulator in the central nervous system (CNS) and the peripheral nervous system (PNS). In the present paper, we first compared three real-time methods used to measure noradrenaline secretion in single cells (membrane capacitance, amperometry and confocal fluorescence microscopy imaging). Compared to the electrophysiological method and fluorescence microscopy, the basic usage of the carbon fiber electrode (CFE) in neuroscience research was presented as an example. Then, we presented a primary description of ion channels, including voltage-gated Na(+), K(+) and Ca(2+) channels in locus coeruleus (LC) neurons in rat brain slices. Finally, we presented example recordings of combined patch-clamp and amperometry measurements in LC neurons, indicating Ca(2+)-dependent quantal noradrenaline release following Ca(2+) influx through Ca(2+) channels.
AB - In order to investigate the central nervous mechanism and the diseases involved in catecholamine transmitter secretion, the dynamics of catecholamine release is studied in single cell, brain slice or in vivo. Noradrenaline is an important neurotransmitter and modulator in the central nervous system (CNS) and the peripheral nervous system (PNS). In the present paper, we first compared three real-time methods used to measure noradrenaline secretion in single cells (membrane capacitance, amperometry and confocal fluorescence microscopy imaging). Compared to the electrophysiological method and fluorescence microscopy, the basic usage of the carbon fiber electrode (CFE) in neuroscience research was presented as an example. Then, we presented a primary description of ion channels, including voltage-gated Na(+), K(+) and Ca(2+) channels in locus coeruleus (LC) neurons in rat brain slices. Finally, we presented example recordings of combined patch-clamp and amperometry measurements in LC neurons, indicating Ca(2+)-dependent quantal noradrenaline release following Ca(2+) influx through Ca(2+) channels.
UR - http://www.scopus.com/inward/record.url?scp=48749130659&partnerID=8YFLogxK
M3 - Article
C2 - 18157483
AN - SCOPUS:48749130659
SN - 0371-0874
VL - 59
SP - 865
EP - 870
JO - Sheng li xue bao : [Acta physiologica Sinica]
JF - Sheng li xue bao : [Acta physiologica Sinica]
IS - 6
ER -