TY - JOUR
T1 - Rapid establishment of p-nitrophenol biodegradation in acetate-fed aerobic granular sludge
AU - Yarlagadda, Venkata Nancharaiah
AU - Kadali, Renu
AU - Sharma, Naresh
AU - Sekar, Raju
AU - Purath, Venugopalan Vayalam
PY - 2012/3
Y1 - 2012/3
N2 - The aim of the study was to investigate the acclimation of precultivated acetatefed aerobic granular sludge to a toxic xenobiotic biodegradation. Establishment of p-nitrophenol (PNP) biodegradation in acetate-fed aerobic granular sludge and concomitant changes in the microstructure and bacterial community were determined. Rapid establishment of PNP utilization was observed in the granular sludge when fed with PNP as the sole carbon source. The specific PNP removal was 36-mg h -1 g -1 granular biomass at an initial PNP concentration of 50 mg L -1. The presence of PNP resulted in significant membrane damage in a subpopulation of the bacterial consortium, as shown by BacLight viability staining. This was coincided with a significant decrease in the culturable bacterial diversity of the granular biomass. PCR-DGGE analysis revealed a shift and decrease in number of bands during the establishment of PNP biodegradation. Scanning electron microscopy showed the dominance of rod-shaped bacteria in the PNP-utilizing microbial granules. Our results suggest that acetate-fed granular sludge could be quickly adapted for PNP biodegradation.
AB - The aim of the study was to investigate the acclimation of precultivated acetatefed aerobic granular sludge to a toxic xenobiotic biodegradation. Establishment of p-nitrophenol (PNP) biodegradation in acetate-fed aerobic granular sludge and concomitant changes in the microstructure and bacterial community were determined. Rapid establishment of PNP utilization was observed in the granular sludge when fed with PNP as the sole carbon source. The specific PNP removal was 36-mg h -1 g -1 granular biomass at an initial PNP concentration of 50 mg L -1. The presence of PNP resulted in significant membrane damage in a subpopulation of the bacterial consortium, as shown by BacLight viability staining. This was coincided with a significant decrease in the culturable bacterial diversity of the granular biomass. PCR-DGGE analysis revealed a shift and decrease in number of bands during the establishment of PNP biodegradation. Scanning electron microscopy showed the dominance of rod-shaped bacteria in the PNP-utilizing microbial granules. Our results suggest that acetate-fed granular sludge could be quickly adapted for PNP biodegradation.
KW - Aerobic granular sludge
KW - Aerobic granules
KW - Biodegradation
KW - p-nitrophenol
UR - http://www.scopus.com/inward/record.url?scp=84858702727&partnerID=8YFLogxK
U2 - 10.1007/s12010-011-9509-3
DO - 10.1007/s12010-011-9509-3
M3 - Article
C2 - 22205323
AN - SCOPUS:84858702727
SN - 0273-2289
VL - 166
SP - 1225
EP - 1235
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology
IS - 5
ER -