Polymorphism in PGLYRP-1 gene by PCR-RFLP and its association with somatic cell score in Chinese Holstein

H. L. Wang; Z. X. Li; L. J. Wang; H. He; J. Yang; L. Chen; F. B. Niu; Y. Liu; J. Z. Guo; X. L. Liu

doi:10.1016/j.rvsc.2013.06.005

Polymorphism in PGLYRP-1 gene by PCR-RFLP and its association with somatic cell score in Chinese Holstein

H. L. Wang, Z. X. Li, L. J. Wang, H. He, J. Yang, L. Chen, F. B. Niu, Y. Liu, J. Z. Guo, X. L. Liu^*

^*Corresponding author for this work

Northwest Agriculture and Forestry University

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Bovine peptidoglycan recognition protein 1 (PGLYRP-1), an important pattern recognition molecule (PRM) of the innate immune system, is an effector molecule in killing different microorganisms directly. To investigate whether the PGLYRP-1 gene was associated with mastitis and milk production traits in dairy cattle, the polymorphism of this gene was analyzed by PCR-RFLP in a population of 524 Chinese Holstein. A total of ten single nucleotide polymorphism (SNP) loci were identified. The association analysis of single SNP locus showed that T. - 35A, T. - 12G and G. +. 102C were significantly associated (P<0.05) with somatic cell score (SCS), while G. +. 102C and G. +. 649C were significantly associated (P<0.05) with 305-day milk yield. Association analysis between combined haplotypes and SCS, milk production traits indicated that H3H3 was associated with the lower SCS (P<0.01), and H2H2 was associated with the lower 305-day milk yield (P<0.01). These findings demonstrated that polymorphisms in PGLYRP-1 gene associated with mastitis resistance and 305-day milk yield, and the H3H3 would provide a useful genetic marker of combined haplotypes for mastitis resistance selection and breeding in Chinese Holstein.

Original language	English
Pages (from-to)	508-514
Number of pages	7
Journal	Research in Veterinary Science
Volume	95
Issue number	2
DOIs	https://doi.org/10.1016/j.rvsc.2013.06.005
Publication status	Published - Oct 2013
Externally published	Yes

Keywords

Mastitis
PCR-PIRA
PCR-RFLP
PGLYRP-1 gene
Polymorphism
SCC

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10.1016/j.rvsc.2013.06.005

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@article{af9fdcfe8206430c8e22725a0673fc17,

title = "Polymorphism in PGLYRP-1 gene by PCR-RFLP and its association with somatic cell score in Chinese Holstein",

abstract = "Bovine peptidoglycan recognition protein 1 (PGLYRP-1), an important pattern recognition molecule (PRM) of the innate immune system, is an effector molecule in killing different microorganisms directly. To investigate whether the PGLYRP-1 gene was associated with mastitis and milk production traits in dairy cattle, the polymorphism of this gene was analyzed by PCR-RFLP in a population of 524 Chinese Holstein. A total of ten single nucleotide polymorphism (SNP) loci were identified. The association analysis of single SNP locus showed that T. - 35A, T. - 12G and G. +. 102C were significantly associated (P<0.05) with somatic cell score (SCS), while G. +. 102C and G. +. 649C were significantly associated (P<0.05) with 305-day milk yield. Association analysis between combined haplotypes and SCS, milk production traits indicated that H3H3 was associated with the lower SCS (P<0.01), and H2H2 was associated with the lower 305-day milk yield (P<0.01). These findings demonstrated that polymorphisms in PGLYRP-1 gene associated with mastitis resistance and 305-day milk yield, and the H3H3 would provide a useful genetic marker of combined haplotypes for mastitis resistance selection and breeding in Chinese Holstein.",

keywords = "Mastitis, PCR-PIRA, PCR-RFLP, PGLYRP-1 gene, Polymorphism, SCC",

author = "Wang, {H. L.} and Li, {Z. X.} and Wang, {L. J.} and H. He and J. Yang and L. Chen and Niu, {F. B.} and Y. Liu and Guo, {J. Z.} and Liu, {X. L.}",

note = "Funding Information: This study was supported by the National “863” Program of China (No. 2008AA101010), the National Science and Technology Major Project of the Ministry of Science and Technology of China during the “12th Five-Year Plan” (No. 2011AA100307), and “13115” Sci-Tech Innovation Program of Shaanxi Province (No. 2008ZDKG-11).",

year = "2013",

month = oct,

doi = "10.1016/j.rvsc.2013.06.005",

language = "English",

volume = "95",

pages = "508--514",

journal = "Research in Veterinary Science",

issn = "0034-5288",

number = "2",

}

TY - JOUR

T1 - Polymorphism in PGLYRP-1 gene by PCR-RFLP and its association with somatic cell score in Chinese Holstein

AU - Wang, H. L.

AU - Li, Z. X.

AU - Wang, L. J.

AU - He, H.

AU - Yang, J.

AU - Chen, L.

AU - Niu, F. B.

AU - Liu, Y.

AU - Guo, J. Z.

AU - Liu, X. L.

N1 - Funding Information: This study was supported by the National “863” Program of China (No. 2008AA101010), the National Science and Technology Major Project of the Ministry of Science and Technology of China during the “12th Five-Year Plan” (No. 2011AA100307), and “13115” Sci-Tech Innovation Program of Shaanxi Province (No. 2008ZDKG-11).

PY - 2013/10

Y1 - 2013/10

N2 - Bovine peptidoglycan recognition protein 1 (PGLYRP-1), an important pattern recognition molecule (PRM) of the innate immune system, is an effector molecule in killing different microorganisms directly. To investigate whether the PGLYRP-1 gene was associated with mastitis and milk production traits in dairy cattle, the polymorphism of this gene was analyzed by PCR-RFLP in a population of 524 Chinese Holstein. A total of ten single nucleotide polymorphism (SNP) loci were identified. The association analysis of single SNP locus showed that T. - 35A, T. - 12G and G. +. 102C were significantly associated (P<0.05) with somatic cell score (SCS), while G. +. 102C and G. +. 649C were significantly associated (P<0.05) with 305-day milk yield. Association analysis between combined haplotypes and SCS, milk production traits indicated that H3H3 was associated with the lower SCS (P<0.01), and H2H2 was associated with the lower 305-day milk yield (P<0.01). These findings demonstrated that polymorphisms in PGLYRP-1 gene associated with mastitis resistance and 305-day milk yield, and the H3H3 would provide a useful genetic marker of combined haplotypes for mastitis resistance selection and breeding in Chinese Holstein.

AB - Bovine peptidoglycan recognition protein 1 (PGLYRP-1), an important pattern recognition molecule (PRM) of the innate immune system, is an effector molecule in killing different microorganisms directly. To investigate whether the PGLYRP-1 gene was associated with mastitis and milk production traits in dairy cattle, the polymorphism of this gene was analyzed by PCR-RFLP in a population of 524 Chinese Holstein. A total of ten single nucleotide polymorphism (SNP) loci were identified. The association analysis of single SNP locus showed that T. - 35A, T. - 12G and G. +. 102C were significantly associated (P<0.05) with somatic cell score (SCS), while G. +. 102C and G. +. 649C were significantly associated (P<0.05) with 305-day milk yield. Association analysis between combined haplotypes and SCS, milk production traits indicated that H3H3 was associated with the lower SCS (P<0.01), and H2H2 was associated with the lower 305-day milk yield (P<0.01). These findings demonstrated that polymorphisms in PGLYRP-1 gene associated with mastitis resistance and 305-day milk yield, and the H3H3 would provide a useful genetic marker of combined haplotypes for mastitis resistance selection and breeding in Chinese Holstein.

KW - Mastitis

KW - PCR-PIRA

KW - PCR-RFLP

KW - PGLYRP-1 gene

KW - Polymorphism

KW - SCC

UR - http://www.scopus.com/inward/record.url?scp=84881542114&partnerID=8YFLogxK

U2 - 10.1016/j.rvsc.2013.06.005

DO - 10.1016/j.rvsc.2013.06.005

M3 - Article

C2 - 23820447

AN - SCOPUS:84881542114

SN - 0034-5288

VL - 95

SP - 508

EP - 514

JO - Research in Veterinary Science

JF - Research in Veterinary Science

IS - 2

ER -

Polymorphism in PGLYRP-1 gene by PCR-RFLP and its association with somatic cell score in Chinese Holstein

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