TY - GEN
T1 - PEG-PLGA nanoparticle modified by transferrin loading doxorubicin
T2 - 3rd International Conference on Advanced Engineering Materials and Technology, AEMT 2013
AU - Liu, Guo Dong
AU - Mao, Jin Ning
AU - Sun, Tao
AU - Jiang, Zhen
AU - Dong, Jun
AU - Huang, Qiang
AU - Lan, Qing
PY - 2013
Y1 - 2013
N2 - Purpose A biodegradable PEG-PLGA nanoparticle(NP) modified by transferrin (Tf) was conceived. Doxorubicin (Dox), a widely used antitumor agent, without passing through the BBB, which limited its utility on glioma, was encapsulated inside (Tf-NP-Dox). Furthermore, its therapeutic efficacy to glioma was evaluated both in vitro and in vivo. Methods Tf-NP-Dox was prepared via modified single emulsion method. Its characterization including size, Drug loading capacity (DLC), entrapment efficiency (EE), Tf number on Tf-NP-Dox surface were estimated. The antitumor efficiency in vitro was evaluated via MTT assay. The transmembrane transportation was evaluated via HPLC assay. The antitumor efficiency in vivo was assessed on C6 glioma intracranial implant rats model. Results The average diameter of Tf-NP-Dox is around 200 nm with surface Tf molecule number per Tf-NP-Dox approximately 25. MTT assay demonstrated stronger cytotoxicity of Tf-NP-Dox to C6 glioma cells (P<0.01). HPLC assay showed Tf-NP-Dox transport Dox into C6 with higher efficiency compare to NP-Dox or Dox (P<0.01). On C6 glioma bearing rat, Tf-NP-Dox could transport more Dox into tumors tested by HPLC assay (P<0.05), and extended life span markedly compared to NP-Dox or Dox (P<0.05). Conclusions Tf-NP-Dox had a potential of glioma targeting and had a better therapeutic effect to glioma both in vitro and in vivo.
AB - Purpose A biodegradable PEG-PLGA nanoparticle(NP) modified by transferrin (Tf) was conceived. Doxorubicin (Dox), a widely used antitumor agent, without passing through the BBB, which limited its utility on glioma, was encapsulated inside (Tf-NP-Dox). Furthermore, its therapeutic efficacy to glioma was evaluated both in vitro and in vivo. Methods Tf-NP-Dox was prepared via modified single emulsion method. Its characterization including size, Drug loading capacity (DLC), entrapment efficiency (EE), Tf number on Tf-NP-Dox surface were estimated. The antitumor efficiency in vitro was evaluated via MTT assay. The transmembrane transportation was evaluated via HPLC assay. The antitumor efficiency in vivo was assessed on C6 glioma intracranial implant rats model. Results The average diameter of Tf-NP-Dox is around 200 nm with surface Tf molecule number per Tf-NP-Dox approximately 25. MTT assay demonstrated stronger cytotoxicity of Tf-NP-Dox to C6 glioma cells (P<0.01). HPLC assay showed Tf-NP-Dox transport Dox into C6 with higher efficiency compare to NP-Dox or Dox (P<0.01). On C6 glioma bearing rat, Tf-NP-Dox could transport more Dox into tumors tested by HPLC assay (P<0.05), and extended life span markedly compared to NP-Dox or Dox (P<0.05). Conclusions Tf-NP-Dox had a potential of glioma targeting and had a better therapeutic effect to glioma both in vitro and in vivo.
KW - Biodegradable nanoparticle
KW - Chemotherapy
KW - Doxorubicin
KW - Glioma
KW - Transferrin
UR - http://www.scopus.com/inward/record.url?scp=84884896070&partnerID=8YFLogxK
U2 - 10.4028/www.scientific.net/AMR.750-752.1643
DO - 10.4028/www.scientific.net/AMR.750-752.1643
M3 - Conference Proceeding
AN - SCOPUS:84884896070
SN - 9783037857632
T3 - Advanced Materials Research
SP - 1643
EP - 1650
BT - Advanced Engineering Materials III
Y2 - 11 May 2013 through 12 May 2013
ER -