Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen receptor-redirected T cells

E. Elkord; D. J. Burt

doi:10.1111/j.1365-3083.2011.02588.x

Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen receptor-redirected T cells

E. Elkord^*, D. J. Burt

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

We here describe the development of a novel ELISPOT assay for the detection and enumeration of IFNγ-secreting functional chimeric antigen receptor (CAR)-redirected T cells against carcinoembryonic antigen (CEA). This method is valuable for clinical trials to monitor the presence of functional CEA-specific T cells transduced with a CAR. The same principle should be applicable for the detection of functional CAR-redirected T cells against any other tumour-associated antigens by immobilizing a particular biotinylated antigen to streptavidin-coated beads.

Original language	English
Pages (from-to)	419-422
Number of pages	4
Journal	Scandinavian Journal of Immunology
Volume	74
Issue number	4
DOIs	https://doi.org/10.1111/j.1365-3083.2011.02588.x
Publication status	Published - Oct 2011
Externally published	Yes

Access to Document

10.1111/j.1365-3083.2011.02588.x

Cite this

@article{0a0e2d9acac84e729405c4dd38e95cc1,

title = "Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen receptor-redirected T cells",

abstract = "We here describe the development of a novel ELISPOT assay for the detection and enumeration of IFNγ-secreting functional chimeric antigen receptor (CAR)-redirected T cells against carcinoembryonic antigen (CEA). This method is valuable for clinical trials to monitor the presence of functional CEA-specific T cells transduced with a CAR. The same principle should be applicable for the detection of functional CAR-redirected T cells against any other tumour-associated antigens by immobilizing a particular biotinylated antigen to streptavidin-coated beads.",

author = "E. Elkord and Burt, {D. J.}",

year = "2011",

month = oct,

doi = "10.1111/j.1365-3083.2011.02588.x",

language = "English",

volume = "74",

pages = "419--422",

journal = "Scandinavian Journal of Immunology",

issn = "0300-9475",

number = "4",

}

TY - JOUR

T1 - Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen receptor-redirected T cells

AU - Elkord, E.

AU - Burt, D. J.

PY - 2011/10

Y1 - 2011/10

N2 - We here describe the development of a novel ELISPOT assay for the detection and enumeration of IFNγ-secreting functional chimeric antigen receptor (CAR)-redirected T cells against carcinoembryonic antigen (CEA). This method is valuable for clinical trials to monitor the presence of functional CEA-specific T cells transduced with a CAR. The same principle should be applicable for the detection of functional CAR-redirected T cells against any other tumour-associated antigens by immobilizing a particular biotinylated antigen to streptavidin-coated beads.

AB - We here describe the development of a novel ELISPOT assay for the detection and enumeration of IFNγ-secreting functional chimeric antigen receptor (CAR)-redirected T cells against carcinoembryonic antigen (CEA). This method is valuable for clinical trials to monitor the presence of functional CEA-specific T cells transduced with a CAR. The same principle should be applicable for the detection of functional CAR-redirected T cells against any other tumour-associated antigens by immobilizing a particular biotinylated antigen to streptavidin-coated beads.

UR - http://www.scopus.com/inward/record.url?scp=80052691211&partnerID=8YFLogxK

U2 - 10.1111/j.1365-3083.2011.02588.x

DO - 10.1111/j.1365-3083.2011.02588.x

M3 - Article

C2 - 21645031

AN - SCOPUS:80052691211

SN - 0300-9475

VL - 74

SP - 419

EP - 422

JO - Scandinavian Journal of Immunology

JF - Scandinavian Journal of Immunology

IS - 4

ER -

Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen receptor-redirected T cells

Abstract

Access to Document

Other files and links

Fingerprint

Cite this