Neurophenotyping of adult zebrafish using the light/dark box paradigm

Adam Stewart; Caio Maximino; Thiago Marques De Brito; Anderson Manoel Herculano; Amauri Gouveia; Silvio Morato; Jonathan M. Cachat; Siddharth Gaikwad; Marco F. Elegante; Peter C. Hart; Allan V. Kalueff

doi:10.1007/978-1-60761-953-6_13

Neurophenotyping of adult zebrafish using the light/dark box paradigm

Adam Stewart, Caio Maximino, Thiago Marques De Brito, Anderson Manoel Herculano, Amauri Gouveia, Silvio Morato, Jonathan M. Cachat, Siddharth Gaikwad, Marco F. Elegante, Peter C. Hart, Allan V. Kalueff

Research output: Chapter in Book or Report/Conference proceeding › Chapter › peer-review

65 Citations (Scopus)

Abstract

The light/dark box test, traditionally used to quantify rodent anxiety-like behavior, has recently been applied to the adult zebrafish (Danio rerio). Utilizing the fish's scototaxis (aversion to bright areas and natural preference for the dark), this paradigm can be used to assess levels of anxiety in adult zebrafish. The light/dark box is a simple and time-efficient one-trial test that does not require pre-training the animals. Importantly, this novelty-based paradigm may also represent a useful tool for studying the pharmacological modulation of zebrafish behavior. Summarizing the experience with this model in several laboratories, here we outline a protocol for the neurophenoptyping of zebrafish anxiety-like behavior using the light/dark paradigm.

Original language	English
Title of host publication	Zebrafish Neurobehavioral Protocols
Editors	Allan Kalueff, Jonathan Cachat
Pages	157-167
Number of pages	11
DOIs	https://doi.org/10.1007/978-1-60761-953-6_13
Publication status	Published - 2011
Externally published	Yes

Publication series

Name	Neuromethods
Volume	51
ISSN (Print)	0893-2336
ISSN (Electronic)	1940-6045

Keywords

Anxiety
Light/Dark box
Novelty-based paradigm
Scototaxis
Zebrafish

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-60761-953-6_13

Cite this

Stewart, A., Maximino, C., De Brito, T. M., Herculano, A. M., Gouveia, A., Morato, S., Cachat, J. M., Gaikwad, S., Elegante, M. F., Hart, P. C., & Kalueff, A. V. (2011). Neurophenotyping of adult zebrafish using the light/dark box paradigm. In A. Kalueff, & J. Cachat (Eds.), Zebrafish Neurobehavioral Protocols (pp. 157-167). (Neuromethods; Vol. 51). https://doi.org/10.1007/978-1-60761-953-6_13

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abstract = "The light/dark box test, traditionally used to quantify rodent anxiety-like behavior, has recently been applied to the adult zebrafish (Danio rerio). Utilizing the fish's scototaxis (aversion to bright areas and natural preference for the dark), this paradigm can be used to assess levels of anxiety in adult zebrafish. The light/dark box is a simple and time-efficient one-trial test that does not require pre-training the animals. Importantly, this novelty-based paradigm may also represent a useful tool for studying the pharmacological modulation of zebrafish behavior. Summarizing the experience with this model in several laboratories, here we outline a protocol for the neurophenoptyping of zebrafish anxiety-like behavior using the light/dark paradigm.",

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AU - Stewart, Adam

AU - Maximino, Caio

AU - De Brito, Thiago Marques

AU - Herculano, Anderson Manoel

AU - Gouveia, Amauri

AU - Morato, Silvio

AU - Cachat, Jonathan M.

AU - Gaikwad, Siddharth

AU - Elegante, Marco F.

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AB - The light/dark box test, traditionally used to quantify rodent anxiety-like behavior, has recently been applied to the adult zebrafish (Danio rerio). Utilizing the fish's scototaxis (aversion to bright areas and natural preference for the dark), this paradigm can be used to assess levels of anxiety in adult zebrafish. The light/dark box is a simple and time-efficient one-trial test that does not require pre-training the animals. Importantly, this novelty-based paradigm may also represent a useful tool for studying the pharmacological modulation of zebrafish behavior. Summarizing the experience with this model in several laboratories, here we outline a protocol for the neurophenoptyping of zebrafish anxiety-like behavior using the light/dark paradigm.

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ER -

Neurophenotyping of adult zebrafish using the light/dark box paradigm

Abstract

Publication series

Keywords

UN SDGs

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