@article{421e17d5b7824be8b92f70faf58d8d8a,
title = "Multi-site assessment of the precision and reproducibility of multiple reaction monitoring-based measurements of proteins in plasma",
abstract = "Verification of candidate biomarkers relies upon specific, quantitative assays optimized for selective detection of target proteins, and is increasingly viewed as a critical step in the discovery pipeline that bridges unbiased biomarker discovery to preclinical validation. Although individual laboratories have demonstrated that multiple reaction monitoring (MRM) coupled with isotope dilution mass spectrometry can quantify candidate protein biomarkers in plasma, reproducibility and transferability of these assays between laboratories have not been demonstrated. We describe a multilaboratory study to assess reproducibility, recovery, linear dynamic range and limits of detection and quantification of multiplexed, MRM-based assays, conducted by NCI-CPTAC. Using common materials and standardized protocols, we demonstrate that these assays can be highly reproducible within and across laboratories and instrument platforms, and are sensitive to low g/ml protein concentrations in unfractionated plasma. We provide data and benchmarks against which individual laboratories can compare their performance and evaluate new technologies for biomarker verification in plasma.",
author = "Addona, {Terri A.} and Abbatiello, {Susan E.} and Birgit Schilling and Skates, {Steven J.} and Mani, {D. R.} and Bunk, {David M.} and Spiegelman, {Clifford H.} and Zimmerman, {Lisa J.} and Ham, {Amy Joan L.} and Hasmik Keshishian and Hall, {Steven C.} and Simon Allen and Blackman, {Ronald K.} and Borchers, {Christoph H.} and Charles Buck and Cardasis, {Helene L.} and Cusack, {Michael P.} and Dodder, {Nathan G.} and Gibson, {Bradford W.} and Held, {Jason M.} and Tara Hiltke and Angela Jackson and Johansen, {Eric B.} and Kinsinger, {Christopher R.} and Jing Li and Mehdi Mesri and Neubert, {Thomas A.} and Niles, {Richard K.} and Pulsipher, {Trenton C.} and David Ransohoff and Henry Rodriguez and Rudnick, {Paul A.} and Derek Smith and Tabb, {David L.} and Tegeler, {Tony J.} and Variyath, {Asokan M.} and Vega-Montoto, {Lorenzo J.} and {\AA}sa Wahlander and Sofia Waldemarson and Mu Wang and Whiteaker, {Jeffrey R.} and Lei Zhao and Anderson, {N. Leigh} and Fisher, {Susan J.} and Liebler, {Daniel C.} and Paulovich, {Amanda G.} and Regnier, {Fred E.} and Paul Tempst and Carr, {Steven A.}",
note = "Funding Information: In the version of this article initially published, the following acknowledgment was inadvertently left out: “The UCSF CPTAC team gratefully acknowledges the support of the Canary Foundation for providing funds to purchase a 4000 QTRAP mass spectrometer.” The acknowlegment has been added to the HTML and PDF versions of the article. Funding Information: This work was supported by grants from the National Cancer Institute (NCI) (U24 CA126476, U24 126477, U24 126480, U24 CA126485, and U24 126479), part of NCI Clinical Proteomic Technologies for Cancer initiative. A component of this initiative is the Clinical Proteomic Technology Assessment for Cancer (CPTAC) Network and teams, which include the Broad Institute of MIT and Harvard (with the Fred Hutchinson Cancer Research Center, Massachusetts General Hospital, the University of North Carolina at Chapel Hill, the University of Victoria and the Plasma Proteome Institute), Memorial Sloan-Kettering Cancer Center (with the Skirball Institute at New York University), Purdue University (with Monarch Life Sciences, Indiana University, Indiana University-Purdue University Indianapolis and the Hoosier Oncology Group), University of California, San Francisco (with the Buck Institute for Age Research, Lawrence Berkeley National Laboratory, the University of British Columbia and the University of Texas M.D. Anderson Cancer Center) and Vanderbilt University School of Medicine (with the University of Texas M.D. Anderson Cancer Center, the University of Washington and the University of Arizona). A full listing of the CPTAC Team Network can be found at http://proteomics.cancer.gov/programs/CPTAC/networkmembership. The UCSF CPTAC team gratefully acknowledges the support of the Canary Foundation for providing funds to purchase a 4000 QTRAP mass spectrometer.",
year = "2009",
month = jul,
doi = "10.1038/nbt.1546",
language = "English",
volume = "27",
pages = "633--641",
journal = "Nature Biotechnology",
issn = "1087-0156",
number = "7",
}