TY - JOUR
T1 - Misexpression of mouse porcupine isoforms modulates the differentiation of P19 embryonic carcinoma cells
AU - Tanaka, Kimiko
AU - Kitagawa, Yasuo
AU - Kadowaki, Tatsuhiko
N1 - Funding Information:
This work was supported by a Research Fellowship from the Japan Society for the Promotion of Science for Young Scientists (to K.T.) and Grant-in-Aids for Scientific Research from the Japan Society for the Promotion of Science (to Y.K. and T.K.). We thank K. Miki for his help in culturing the P19 cells, and K. Matsumoto and J. Tsuji for kindly donating the antibodies to Phospho-SAPK/JNK and SAPK/JNK.
PY - 2003/7/1
Y1 - 2003/7/1
N2 - Drosophila porcupine (porc) encodes an ER membrane protein that is required for the processing of the Drosophila Wnt family. Homologs of porc have been identified in various multicellular organisms and have been implicated in the biosynthesis of Wnt proteins. In contrast to Drosophila, vertebrates generate four different porc mRNAs (A-D) by alternative splicing. Murine porcD (MporcD) mRNA levels transiently increase during the neuroectodermal differentiation of P19 cells, but diminish during mesodermal differentiation. P19 cells constitutively expressing mouse porcA (MporcA), but not MporcD, undergo apoptosis by the induction of neuroectodermal differentiation. Meanwhile, P19 cells constitutively expressing MporcD, but not MporcA, do not adopt mesodermal cell morphology and fail to express myf-5 when induced to mesodermal differentiation. These results therefore demonstrate that the alternative splicing of Mporc is regulated in a cell-type specific manner, and the resulting Mporc isoforms have different functions in the neuroectodermal and mesodermal differentiation of P19 cells.
AB - Drosophila porcupine (porc) encodes an ER membrane protein that is required for the processing of the Drosophila Wnt family. Homologs of porc have been identified in various multicellular organisms and have been implicated in the biosynthesis of Wnt proteins. In contrast to Drosophila, vertebrates generate four different porc mRNAs (A-D) by alternative splicing. Murine porcD (MporcD) mRNA levels transiently increase during the neuroectodermal differentiation of P19 cells, but diminish during mesodermal differentiation. P19 cells constitutively expressing mouse porcA (MporcA), but not MporcD, undergo apoptosis by the induction of neuroectodermal differentiation. Meanwhile, P19 cells constitutively expressing MporcD, but not MporcA, do not adopt mesodermal cell morphology and fail to express myf-5 when induced to mesodermal differentiation. These results therefore demonstrate that the alternative splicing of Mporc is regulated in a cell-type specific manner, and the resulting Mporc isoforms have different functions in the neuroectodermal and mesodermal differentiation of P19 cells.
KW - Alternative splicing
KW - Apoptosis
KW - P19 embryonic carcinoma cells
KW - Porcupine
KW - Wnt
UR - http://www.scopus.com/inward/record.url?scp=0038723734&partnerID=8YFLogxK
U2 - 10.1016/S1065-6995(03)00080-5
DO - 10.1016/S1065-6995(03)00080-5
M3 - Article
C2 - 12842094
AN - SCOPUS:0038723734
SN - 1065-6995
VL - 27
SP - 549
EP - 557
JO - Cell Biology International
JF - Cell Biology International
IS - 7
ER -