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Abstract
Cortisol is a well-known stress biomarker. This study focuses on using electrochemical
immuno-sensing to measure the concentration of cortisol selectively and sensitively in
artificial sample. Anti-cortisol antibody has been immobilised on polycrystalline Au
electrodes via strong covalent thiol bonds, fabricating an electrochemical bioimmunosensor
for cortisol detection. IrOx was anodically electrodeposited as a
reference electrode on a commercial screen-printed electrode. Electrochemical
impedance spectrometry (EIS) studies were used to correlate the electrochemical
response to cortisol concentration and the induced changes in charge transfer
resistance (Rct). A linear relationship between the Rct and the logarithm of cortisol
concentrations were found in the concentrations ranging from 1 ng/mL to 1 mg/mL. The
modification of reference electrode with iridium oxide has greatly improved the
reproducibility of the screen-printed electrode. The sensing system can provide a
reliable and sensitive detection approach for cortisol measurements.
immuno-sensing to measure the concentration of cortisol selectively and sensitively in
artificial sample. Anti-cortisol antibody has been immobilised on polycrystalline Au
electrodes via strong covalent thiol bonds, fabricating an electrochemical bioimmunosensor
for cortisol detection. IrOx was anodically electrodeposited as a
reference electrode on a commercial screen-printed electrode. Electrochemical
impedance spectrometry (EIS) studies were used to correlate the electrochemical
response to cortisol concentration and the induced changes in charge transfer
resistance (Rct). A linear relationship between the Rct and the logarithm of cortisol
concentrations were found in the concentrations ranging from 1 ng/mL to 1 mg/mL. The
modification of reference electrode with iridium oxide has greatly improved the
reproducibility of the screen-printed electrode. The sensing system can provide a
reliable and sensitive detection approach for cortisol measurements.
| Original language | English |
|---|---|
| Pages (from-to) | 126776 |
| Number of pages | 10 |
| Journal | Talanta |
| Volume | 280 |
| Issue number | 1 December 2024 |
| DOIs | |
| Publication status | Published - 24 Aug 2024 |
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Dive into the research topics of 'Iridium Oxide-modified Reference Screen-printed Electrodes for Point-of-Care Portable Electrochemical Cortisol Detection'. Together they form a unique fingerprint.Projects
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The High-temperature enabled metal oxide-based nanomaterials for both glucose sensing and the solid-state pH sensing
Dong, Q., Li, B. & Li, M.
1/03/22 → 1/03/25
Project: Internal Research Project