Kaplan, M., Chreifi, G., Metskas, L. A., Liedtke, J., Wood, C. R., Oikonomou, C. M., Nicolas, W. J., Subramanian, P., Zacharoff, L. A., Wang, Y., Chang, Y. W., Beeby, M., Dobro, M. J., Zhu, Y., McBride, M. J., Briegel, A., Shaffer, C. L., & Jensen, G. J. (2021). In situ imaging of bacterial outer membrane projections and associated protein complexes using electron cryo-tomography. eLife, 10, Article e73099. https://doi.org/10.7554/eLife.73099
Kaplan, Mohammed ; Chreifi, Georges ; Metskas, Lauren Ann et al. / In situ imaging of bacterial outer membrane projections and associated protein complexes using electron cryo-tomography. In: eLife. 2021 ; Vol. 10.
@article{9d96db946add42b491ec610d61b170e6,
title = "In situ imaging of bacterial outer membrane projections and associated protein complexes using electron cryo-tomography",
abstract = "The ability to produce outer membrane projections in the form of tubular membrane extensions (MEs) and membrane vesicles (MVs) is a widespread phenomenon among diderm bacteria. Despite this, our knowledge of the ultrastructure of these extensions and their associated protein complexes remains limited. Here, we surveyed the ultrastructure and formation of MEs and MVs, and their associated protein complexes, in tens of thousands of electron cryo-tomograms of ~90 bacterial species that we have collected for various projects over the past 15 years (Jensen lab database), in addition to data generated in the Briegel lab. We identified outer MEs and MVs in 13 diderm bacterial species and classified several major ultrastructures: (1) tubes with a uniform diameter (with or without an internal scaffold), (2) tubes with irregular diameter, (3) tubes with a vesicular dilation at their tip, (4) pearling tubes, (5) connected chains of vesicles (with or without neck-like connectors), (6) budding vesicles and nanopods. We also identified several protein complexes associated with these MEs and MVs which were distributed either randomly or exclusively at the tip. These complexes include a secretin-like structure and a novel crown-shaped structure observed primarily in vesicles from lysed cells. In total, this work helps to characterize the diversity of bacterial membrane projections and lays the groundwork for future research in this field.",
author = "Mohammed Kaplan and Georges Chreifi and Metskas, {Lauren Ann} and Janine Liedtke and Wood, {Cecily R.} and Oikonomou, {Catherine M.} and Nicolas, {William J.} and Poorna Subramanian and Zacharoff, {Lori A.} and Yuhang Wang and Chang, {Yi Wei} and Morgan Beeby and Dobro, {Megan J.} and Yongtao Zhu and McBride, {Mark J.} and Ariane Briegel and Shaffer, {Carrie L.} and Jensen, {Grant J.}",
note = "Publisher Copyright: {\textcopyright} Kaplan et al.",
year = "2021",
month = sep,
doi = "10.7554/eLife.73099",
language = "English",
volume = "10",
journal = "eLife",
issn = "2050-084X",
}
Kaplan, M, Chreifi, G, Metskas, LA, Liedtke, J, Wood, CR, Oikonomou, CM, Nicolas, WJ, Subramanian, P, Zacharoff, LA, Wang, Y, Chang, YW, Beeby, M, Dobro, MJ, Zhu, Y, McBride, MJ, Briegel, A, Shaffer, CL & Jensen, GJ 2021, 'In situ imaging of bacterial outer membrane projections and associated protein complexes using electron cryo-tomography', eLife, vol. 10, e73099. https://doi.org/10.7554/eLife.73099
In situ imaging of bacterial outer membrane projections and associated protein complexes using electron cryo-tomography. / Kaplan, Mohammed; Chreifi, Georges; Metskas, Lauren Ann et al.
In:
eLife, Vol. 10, e73099, 09.2021.
Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - In situ imaging of bacterial outer membrane projections and associated protein complexes using electron cryo-tomography
AU - Kaplan, Mohammed
AU - Chreifi, Georges
AU - Metskas, Lauren Ann
AU - Liedtke, Janine
AU - Wood, Cecily R.
AU - Oikonomou, Catherine M.
AU - Nicolas, William J.
AU - Subramanian, Poorna
AU - Zacharoff, Lori A.
AU - Wang, Yuhang
AU - Chang, Yi Wei
AU - Beeby, Morgan
AU - Dobro, Megan J.
AU - Zhu, Yongtao
AU - McBride, Mark J.
AU - Briegel, Ariane
AU - Shaffer, Carrie L.
AU - Jensen, Grant J.
N1 - Publisher Copyright:
© Kaplan et al.
PY - 2021/9
Y1 - 2021/9
N2 - The ability to produce outer membrane projections in the form of tubular membrane extensions (MEs) and membrane vesicles (MVs) is a widespread phenomenon among diderm bacteria. Despite this, our knowledge of the ultrastructure of these extensions and their associated protein complexes remains limited. Here, we surveyed the ultrastructure and formation of MEs and MVs, and their associated protein complexes, in tens of thousands of electron cryo-tomograms of ~90 bacterial species that we have collected for various projects over the past 15 years (Jensen lab database), in addition to data generated in the Briegel lab. We identified outer MEs and MVs in 13 diderm bacterial species and classified several major ultrastructures: (1) tubes with a uniform diameter (with or without an internal scaffold), (2) tubes with irregular diameter, (3) tubes with a vesicular dilation at their tip, (4) pearling tubes, (5) connected chains of vesicles (with or without neck-like connectors), (6) budding vesicles and nanopods. We also identified several protein complexes associated with these MEs and MVs which were distributed either randomly or exclusively at the tip. These complexes include a secretin-like structure and a novel crown-shaped structure observed primarily in vesicles from lysed cells. In total, this work helps to characterize the diversity of bacterial membrane projections and lays the groundwork for future research in this field.
AB - The ability to produce outer membrane projections in the form of tubular membrane extensions (MEs) and membrane vesicles (MVs) is a widespread phenomenon among diderm bacteria. Despite this, our knowledge of the ultrastructure of these extensions and their associated protein complexes remains limited. Here, we surveyed the ultrastructure and formation of MEs and MVs, and their associated protein complexes, in tens of thousands of electron cryo-tomograms of ~90 bacterial species that we have collected for various projects over the past 15 years (Jensen lab database), in addition to data generated in the Briegel lab. We identified outer MEs and MVs in 13 diderm bacterial species and classified several major ultrastructures: (1) tubes with a uniform diameter (with or without an internal scaffold), (2) tubes with irregular diameter, (3) tubes with a vesicular dilation at their tip, (4) pearling tubes, (5) connected chains of vesicles (with or without neck-like connectors), (6) budding vesicles and nanopods. We also identified several protein complexes associated with these MEs and MVs which were distributed either randomly or exclusively at the tip. These complexes include a secretin-like structure and a novel crown-shaped structure observed primarily in vesicles from lysed cells. In total, this work helps to characterize the diversity of bacterial membrane projections and lays the groundwork for future research in this field.
UR - http://www.scopus.com/inward/record.url?scp=85117147288&partnerID=8YFLogxK
U2 - 10.7554/eLife.73099
DO - 10.7554/eLife.73099
M3 - Article
C2 - 34468314
AN - SCOPUS:85117147288
SN - 2050-084X
VL - 10
JO - eLife
JF - eLife
M1 - e73099
ER -
Kaplan M, Chreifi G, Metskas LA, Liedtke J, Wood CR, Oikonomou CM et al. In situ imaging of bacterial outer membrane projections and associated protein complexes using electron cryo-tomography. eLife. 2021 Sept;10:e73099. doi: 10.7554/eLife.73099