Importing, caring, breeding, genotyping, and phenotyping a genetic mouse in a Chinese University

S. T. Kuo; Q. H. Wu; B. Liu; Z. L. Xie; X. Wu; S. J. Shang; X. Y. Zhang; X. J. Kang; L. N. Liu; F. P. Zhu; Y. S. Wang; M. Q. Hu; H. D. Xu; L. Zhou; B. Liu; Z. Y. Chai; Q. F. Zhang; W. Liu; S. S. Teng; C. H. Wang; N. Guo; H. Q. Dou; P. L. Zuo; L. H. Zheng; C. X. Zhang; D. S. Zhu; L. Wang; S. R. Wang; Z. Zhou

doi:10.1007/s12031-013-0195-1

Importing, caring, breeding, genotyping, and phenotyping a genetic mouse in a Chinese University

S. T. Kuo, Q. H. Wu, B. Liu, Z. L. Xie, X. Wu, S. J. Shang, X. Y. Zhang, X. J. Kang, L. N. Liu, F. P. Zhu, Y. S. Wang, M. Q. Hu, H. D. Xu, L. Zhou, B. Liu, Z. Y. Chai, Q. F. Zhang, W. Liu, S. S. Teng, C. H. WangN. Guo, H. Q. Dou, P. L. Zuo, L. H. Zheng, C. X. Zhang, D. S. Zhu, L. Wang^*, S. R. Wang, Z. Zhou

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The genetic manipulation of the laboratory mouse has been well developed and generated more and more mouse lines for biomedical research. To advance our science exploration, it is necessary to share genetically modified mouse lines with collaborators between institutions, even in different countries. The transfer process is complicated. Significant paperwork and coordination are required, concerning animal welfare, intellectual property rights, colony health status, and biohazard. Here, we provide a practical example of importing a transgenic mice line, Dynamin 1 knockout mice, from Yale University in the USA to Perking University in China for studying cell secretion. This example including the length of time that required for paper work, mice quarantine at the receiving institution, and expansion of the mouse line for experiments. The procedure described in this paper for delivery live transgenic mice from USA to China may serve a simple reference for transferring mouse lines between other countries too.

Original language	English
Pages (from-to)	487-492
Number of pages	6
Journal	Journal of Molecular Neuroscience
Volume	53
Issue number	3
DOIs	https://doi.org/10.1007/s12031-013-0195-1
Publication status	Published - Jun 2014
Externally published	Yes

Keywords

Breeding
Chromaffin cells
Dynamin 1
Genetic mice
Genotyping

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10.1007/s12031-013-0195-1

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Kuo, S. T., Wu, Q. H., Liu, B., Xie, Z. L., Wu, X., Shang, S. J., Zhang, X. Y., Kang, X. J., Liu, L. N., Zhu, F. P., Wang, Y. S., Hu, M. Q., Xu, H. D., Zhou, L., Liu, B., Chai, Z. Y., Zhang, Q. F., Liu, W., Teng, S. S., ... Zhou, Z. (2014). Importing, caring, breeding, genotyping, and phenotyping a genetic mouse in a Chinese University. Journal of Molecular Neuroscience, 53(3), 487-492. https://doi.org/10.1007/s12031-013-0195-1

@article{d15410f592a34fd1bd19533daf0e371d,

title = "Importing, caring, breeding, genotyping, and phenotyping a genetic mouse in a Chinese University",

abstract = "The genetic manipulation of the laboratory mouse has been well developed and generated more and more mouse lines for biomedical research. To advance our science exploration, it is necessary to share genetically modified mouse lines with collaborators between institutions, even in different countries. The transfer process is complicated. Significant paperwork and coordination are required, concerning animal welfare, intellectual property rights, colony health status, and biohazard. Here, we provide a practical example of importing a transgenic mice line, Dynamin 1 knockout mice, from Yale University in the USA to Perking University in China for studying cell secretion. This example including the length of time that required for paper work, mice quarantine at the receiving institution, and expansion of the mouse line for experiments. The procedure described in this paper for delivery live transgenic mice from USA to China may serve a simple reference for transferring mouse lines between other countries too.",

keywords = "Breeding, Chromaffin cells, Dynamin 1, Genetic mice, Genotyping",

author = "Kuo, {S. T.} and Wu, {Q. H.} and B. Liu and Xie, {Z. L.} and X. Wu and Shang, {S. J.} and Zhang, {X. Y.} and Kang, {X. J.} and Liu, {L. N.} and Zhu, {F. P.} and Wang, {Y. S.} and Hu, {M. Q.} and Xu, {H. D.} and L. Zhou and B. Liu and Chai, {Z. Y.} and Zhang, {Q. F.} and W. Liu and Teng, {S. S.} and Wang, {C. H.} and N. Guo and Dou, {H. Q.} and Zuo, {P. L.} and Zheng, {L. H.} and Zhang, {C. X.} and Zhu, {D. S.} and L. Wang and Wang, {S. R.} and Z. Zhou",

note = "Funding Information: Acknowledgements We thank Dr. I.C. Bruce for helpful comments on the manuscript. We thank staffs of the Laboratory Animal Center of Peking University and Beijing Sun-rising Technologies Co. for their excellent services. This work was supported by grants from the National Basic Research Program of China (2007CB512100, 2012CB518006, and 2006CB500800), the National Natural Science Foundation of China (30728009, 30770521, 30770788, 30970660, 30911120491, 30830043, and 31330024).",

year = "2014",

month = jun,

doi = "10.1007/s12031-013-0195-1",

language = "English",

volume = "53",

pages = "487--492",

journal = "Journal of Molecular Neuroscience",

issn = "0895-8696",

number = "3",

}

Kuo, ST, Wu, QH, Liu, B, Xie, ZL, Wu, X, Shang, SJ, Zhang, XY, Kang, XJ, Liu, LN, Zhu, FP, Wang, YS, Hu, MQ, Xu, HD, Zhou, L, Liu, B, Chai, ZY, Zhang, QF, Liu, W, Teng, SS, Wang, CH, Guo, N, Dou, HQ, Zuo, PL, Zheng, LH, Zhang, CX, Zhu, DS, Wang, L, Wang, SR & Zhou, Z 2014, 'Importing, caring, breeding, genotyping, and phenotyping a genetic mouse in a Chinese University', Journal of Molecular Neuroscience, vol. 53, no. 3, pp. 487-492. https://doi.org/10.1007/s12031-013-0195-1

TY - JOUR

T1 - Importing, caring, breeding, genotyping, and phenotyping a genetic mouse in a Chinese University

AU - Kuo, S. T.

AU - Wu, Q. H.

AU - Liu, B.

AU - Xie, Z. L.

AU - Wu, X.

AU - Shang, S. J.

AU - Zhang, X. Y.

AU - Kang, X. J.

AU - Liu, L. N.

AU - Zhu, F. P.

AU - Wang, Y. S.

AU - Hu, M. Q.

AU - Xu, H. D.

AU - Zhou, L.

AU - Liu, B.

AU - Chai, Z. Y.

AU - Zhang, Q. F.

AU - Liu, W.

AU - Teng, S. S.

AU - Wang, C. H.

AU - Guo, N.

AU - Dou, H. Q.

AU - Zuo, P. L.

AU - Zheng, L. H.

AU - Zhang, C. X.

AU - Zhu, D. S.

AU - Wang, L.

AU - Wang, S. R.

AU - Zhou, Z.

N1 - Funding Information: Acknowledgements We thank Dr. I.C. Bruce for helpful comments on the manuscript. We thank staffs of the Laboratory Animal Center of Peking University and Beijing Sun-rising Technologies Co. for their excellent services. This work was supported by grants from the National Basic Research Program of China (2007CB512100, 2012CB518006, and 2006CB500800), the National Natural Science Foundation of China (30728009, 30770521, 30770788, 30970660, 30911120491, 30830043, and 31330024).

PY - 2014/6

Y1 - 2014/6

N2 - The genetic manipulation of the laboratory mouse has been well developed and generated more and more mouse lines for biomedical research. To advance our science exploration, it is necessary to share genetically modified mouse lines with collaborators between institutions, even in different countries. The transfer process is complicated. Significant paperwork and coordination are required, concerning animal welfare, intellectual property rights, colony health status, and biohazard. Here, we provide a practical example of importing a transgenic mice line, Dynamin 1 knockout mice, from Yale University in the USA to Perking University in China for studying cell secretion. This example including the length of time that required for paper work, mice quarantine at the receiving institution, and expansion of the mouse line for experiments. The procedure described in this paper for delivery live transgenic mice from USA to China may serve a simple reference for transferring mouse lines between other countries too.

AB - The genetic manipulation of the laboratory mouse has been well developed and generated more and more mouse lines for biomedical research. To advance our science exploration, it is necessary to share genetically modified mouse lines with collaborators between institutions, even in different countries. The transfer process is complicated. Significant paperwork and coordination are required, concerning animal welfare, intellectual property rights, colony health status, and biohazard. Here, we provide a practical example of importing a transgenic mice line, Dynamin 1 knockout mice, from Yale University in the USA to Perking University in China for studying cell secretion. This example including the length of time that required for paper work, mice quarantine at the receiving institution, and expansion of the mouse line for experiments. The procedure described in this paper for delivery live transgenic mice from USA to China may serve a simple reference for transferring mouse lines between other countries too.

KW - Breeding

KW - Chromaffin cells

KW - Dynamin 1

KW - Genetic mice

KW - Genotyping

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DO - 10.1007/s12031-013-0195-1

M3 - Article

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AN - SCOPUS:84905003939

SN - 0895-8696

VL - 53

SP - 487

EP - 492

JO - Journal of Molecular Neuroscience

JF - Journal of Molecular Neuroscience

IS - 3

ER -

Importing, caring, breeding, genotyping, and phenotyping a genetic mouse in a Chinese University

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Keywords

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