Human serum albumin interaction, in silico and anticancer evaluation of Pine-Gold nanoparticles

K. Anand; R. Rajamanikandan; A. Selva Sharma; M. Ilanchelian; Faez Iqbal Khan; C. Tiloke; Naresh Kumar Katari; P. Boomi; C. Balakumar; M. Saravanan; S. Palanisamy; M. Ramesh; Dakun Lai; A. A. Chuturgoon

doi:10.1016/j.procbio.2019.09.036

Human serum albumin interaction, in silico and anticancer evaluation of Pine-Gold nanoparticles

K. Anand^*, R. Rajamanikandan, A. Selva Sharma, M. Ilanchelian, Faez Iqbal Khan, C. Tiloke, Naresh Kumar Katari, P. Boomi, C. Balakumar, M. Saravanan, S. Palanisamy, M. Ramesh, Dakun Lai, A. A. Chuturgoon

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Unique characteristics displayed by phytoconstituent conjugated nanoparticles and their crucial interactions with proteins serve to develop nanoparticle-bio-interface platform. Gold nanoparticles of 16 nm in size were generated using aqueous extracts of pine bark and further conjugated to human serum albumin. The gold nanoparticles-protein complex was characterized by surface plasmon resonance, UV–vis and emission spectroscopy techniques. Further, it was characterized for surface morphology and elemental composition, crystallographic quality, nanoparticles size, shape, stability, structural determination and the identification of capping agent. Moreover, the interaction of gold nanoparticles with human serum albumin was investigated using conventional spectroscopy techniques. Fluorescence quenching and absorption studies demonstrated an effective binding of human serum albumin with oleamide capped gold nanoparticles. The molecular docking study showed a binding affinity of -6.1 kcal/mol whereas the molecular dynamics simulation indicated that the binding of oleamide to human serum albumin. A biological evaluation of pine bark extract-gold nanoparticles showed cytotoxicity with increased cell mortality in lung cancer cells and minimal toxicity on non-cancerous human embryonic kidney cells, respectively.

Original language	English
Pages (from-to)	98-109
Number of pages	12
Journal	Process Biochemistry
Volume	89
DOIs	https://doi.org/10.1016/j.procbio.2019.09.036
Publication status	Published - Feb 2020
Externally published	Yes

Keywords

Gold nanoparticles
HEK293 cells
Human serum albumin
Oleamide
Pine bark extract

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.procbio.2019.09.036

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Anand, K., Rajamanikandan, R., Selva Sharma, A., Ilanchelian, M., Khan, F. I., Tiloke, C., Katari, N. K., Boomi, P., Balakumar, C., Saravanan, M., Palanisamy, S., Ramesh, M., Lai, D., & Chuturgoon, A. A. (2020). Human serum albumin interaction, in silico and anticancer evaluation of Pine-Gold nanoparticles. Process Biochemistry, 89, 98-109. https://doi.org/10.1016/j.procbio.2019.09.036

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title = "Human serum albumin interaction, in silico and anticancer evaluation of Pine-Gold nanoparticles",

abstract = "Unique characteristics displayed by phytoconstituent conjugated nanoparticles and their crucial interactions with proteins serve to develop nanoparticle-bio-interface platform. Gold nanoparticles of 16 nm in size were generated using aqueous extracts of pine bark and further conjugated to human serum albumin. The gold nanoparticles-protein complex was characterized by surface plasmon resonance, UV–vis and emission spectroscopy techniques. Further, it was characterized for surface morphology and elemental composition, crystallographic quality, nanoparticles size, shape, stability, structural determination and the identification of capping agent. Moreover, the interaction of gold nanoparticles with human serum albumin was investigated using conventional spectroscopy techniques. Fluorescence quenching and absorption studies demonstrated an effective binding of human serum albumin with oleamide capped gold nanoparticles. The molecular docking study showed a binding affinity of -6.1 kcal/mol whereas the molecular dynamics simulation indicated that the binding of oleamide to human serum albumin. A biological evaluation of pine bark extract-gold nanoparticles showed cytotoxicity with increased cell mortality in lung cancer cells and minimal toxicity on non-cancerous human embryonic kidney cells, respectively.",

keywords = "Gold nanoparticles, HEK293 cells, Human serum albumin, Oleamide, Pine bark extract",

author = "K. Anand and R. Rajamanikandan and {Selva Sharma}, A. and M. Ilanchelian and Khan, {Faez Iqbal} and C. Tiloke and Katari, {Naresh Kumar} and P. Boomi and C. Balakumar and M. Saravanan and S. Palanisamy and M. Ramesh and Dakun Lai and Chuturgoon, {A. A.}",

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year = "2020",

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doi = "10.1016/j.procbio.2019.09.036",

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Anand, K, Rajamanikandan, R, Selva Sharma, A, Ilanchelian, M, Khan, FI, Tiloke, C, Katari, NK, Boomi, P, Balakumar, C, Saravanan, M, Palanisamy, S, Ramesh, M, Lai, D & Chuturgoon, AA 2020, 'Human serum albumin interaction, in silico and anticancer evaluation of Pine-Gold nanoparticles', Process Biochemistry, vol. 89, pp. 98-109. https://doi.org/10.1016/j.procbio.2019.09.036

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AU - Anand, K.

AU - Rajamanikandan, R.

AU - Selva Sharma, A.

AU - Ilanchelian, M.

AU - Khan, Faez Iqbal

AU - Tiloke, C.

AU - Katari, Naresh Kumar

AU - Boomi, P.

AU - Balakumar, C.

AU - Saravanan, M.

AU - Palanisamy, S.

AU - Ramesh, M.

AU - Lai, Dakun

AU - Chuturgoon, A. A.

PY - 2020/2

Y1 - 2020/2

N2 - Unique characteristics displayed by phytoconstituent conjugated nanoparticles and their crucial interactions with proteins serve to develop nanoparticle-bio-interface platform. Gold nanoparticles of 16 nm in size were generated using aqueous extracts of pine bark and further conjugated to human serum albumin. The gold nanoparticles-protein complex was characterized by surface plasmon resonance, UV–vis and emission spectroscopy techniques. Further, it was characterized for surface morphology and elemental composition, crystallographic quality, nanoparticles size, shape, stability, structural determination and the identification of capping agent. Moreover, the interaction of gold nanoparticles with human serum albumin was investigated using conventional spectroscopy techniques. Fluorescence quenching and absorption studies demonstrated an effective binding of human serum albumin with oleamide capped gold nanoparticles. The molecular docking study showed a binding affinity of -6.1 kcal/mol whereas the molecular dynamics simulation indicated that the binding of oleamide to human serum albumin. A biological evaluation of pine bark extract-gold nanoparticles showed cytotoxicity with increased cell mortality in lung cancer cells and minimal toxicity on non-cancerous human embryonic kidney cells, respectively.

AB - Unique characteristics displayed by phytoconstituent conjugated nanoparticles and their crucial interactions with proteins serve to develop nanoparticle-bio-interface platform. Gold nanoparticles of 16 nm in size were generated using aqueous extracts of pine bark and further conjugated to human serum albumin. The gold nanoparticles-protein complex was characterized by surface plasmon resonance, UV–vis and emission spectroscopy techniques. Further, it was characterized for surface morphology and elemental composition, crystallographic quality, nanoparticles size, shape, stability, structural determination and the identification of capping agent. Moreover, the interaction of gold nanoparticles with human serum albumin was investigated using conventional spectroscopy techniques. Fluorescence quenching and absorption studies demonstrated an effective binding of human serum albumin with oleamide capped gold nanoparticles. The molecular docking study showed a binding affinity of -6.1 kcal/mol whereas the molecular dynamics simulation indicated that the binding of oleamide to human serum albumin. A biological evaluation of pine bark extract-gold nanoparticles showed cytotoxicity with increased cell mortality in lung cancer cells and minimal toxicity on non-cancerous human embryonic kidney cells, respectively.

KW - Gold nanoparticles

KW - HEK293 cells

KW - Human serum albumin

KW - Oleamide

KW - Pine bark extract

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SP - 98

EP - 109

JO - Process Biochemistry

JF - Process Biochemistry

ER -

Human serum albumin interaction, in silico and anticancer evaluation of Pine-Gold nanoparticles

Abstract

Keywords

UN SDGs

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