TY - JOUR
T1 - Genetic diversity of MYH3 gene associated with growth and carcass traits in Chinese Qinchuan cattle
AU - Niu, Fubiao
AU - Wang, Lijun
AU - Liu, Xiaolin
AU - Wang, Hongliang
AU - Yang, Jing
AU - Liu, Yu
AU - Chen, Ling
N1 - Funding Information:
Acknowledgments Research supported by the National 12th ‘‘Five-Year’’ National Science and Technology Key Project (No.2011A A100307), National 11th ‘‘Five-Year’’ National Science and Technology Key Project (No. 2008AA101010), ‘‘13115’’ Sci-Tech Innovation Program of Shaanxi Province (No.2008ZDKG-11).
PY - 2013/10
Y1 - 2013/10
N2 - MYH3, whose function is to convert chemical energy to mechanical energy through ATP hydrolysis, is mainly expressed in skeletal muscle at various stages and is indispensable in the procedure of development of skeletal muscle and heart. In the study, genetic variations and genotypes of MYH 3 gene in a total of 365 Qinchuan cattles were analyzed by polymerase chain reaction-restriction fragment length polymorphism, as well as verified the effect on growth and carcass traits. After PCR products were digested by restriction enzymes, eight SNPs were identified and individuals were genotyped. It showed that the SNPs at nucleotides were all in lowlinkage disequilibrium, therefore no dominated haplotype was found in the population. The result of statistic analysis indicated seven SNPs were significantly associated with growth and carcass traits (P>0.05, N = 365) except locusG13791A. To sumup, the result of the study proved that polymorphisms inMYH3 gene are associated with the growth performance of Chinese Qinchuan cattle, so the variations of the gene could be used as possible molecular assisted-makers in the beef cattle breeding program and management.
AB - MYH3, whose function is to convert chemical energy to mechanical energy through ATP hydrolysis, is mainly expressed in skeletal muscle at various stages and is indispensable in the procedure of development of skeletal muscle and heart. In the study, genetic variations and genotypes of MYH 3 gene in a total of 365 Qinchuan cattles were analyzed by polymerase chain reaction-restriction fragment length polymorphism, as well as verified the effect on growth and carcass traits. After PCR products were digested by restriction enzymes, eight SNPs were identified and individuals were genotyped. It showed that the SNPs at nucleotides were all in lowlinkage disequilibrium, therefore no dominated haplotype was found in the population. The result of statistic analysis indicated seven SNPs were significantly associated with growth and carcass traits (P>0.05, N = 365) except locusG13791A. To sumup, the result of the study proved that polymorphisms inMYH3 gene are associated with the growth performance of Chinese Qinchuan cattle, so the variations of the gene could be used as possible molecular assisted-makers in the beef cattle breeding program and management.
KW - Genotype
KW - MYH3
KW - PCR-RFLP
KW - Polymorphism
KW - Qinchuan cattle
UR - http://www.scopus.com/inward/record.url?scp=84892722608&partnerID=8YFLogxK
U2 - 10.1007/s11033-013-2665-5
DO - 10.1007/s11033-013-2665-5
M3 - Article
C2 - 24065521
AN - SCOPUS:84892722608
SN - 0301-4851
VL - 40
SP - 5635
EP - 5643
JO - Molecular Biology Reports
JF - Molecular Biology Reports
IS - 10
ER -