Digital imaging as a detector for quantitative colorimetric analyses

L. Byrne, K. Lau, S. Edwards, D. Diamond

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Abstract

In this paper, a digital imaging system was used to analyze a series of colorimetric reagent dots. A commercially available digital camera was used with in-house developed software to capture and analyze data, The color captured was digitized and broken down into three 8-bit color values (red, green and blue). The data was then processed to generate distinct recognizable patterns that represent the original color input. A pH indicator sensor array was used to demonstrate the practicality of the system. Preliminary results show that the system is able to discriminate samples over a wide pH range (pH 1-13) and resolve samples to within ± 0.5 pH units.

Original languageEnglish
Pages (from-to)267-277
Number of pages11
JournalProceedings of SPIE- The International Society for Optical Engineering
Volume4205
DOIs
Publication statusPublished - 2001
Externally publishedYes

Keywords

  • Colorimetric reagent
  • Digital imaging
  • Dot
  • High-density arrays
  • PH
  • Pattern-matching

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Byrne, L., Lau, K., Edwards, S., & Diamond, D. (2001). Digital imaging as a detector for quantitative colorimetric analyses. Proceedings of SPIE- The International Society for Optical Engineering, 4205, 267-277. https://doi.org/10.1117/12.417460