TY - JOUR
T1 - Differentiation of human bone marrow-derived mesenchymal stem cells into neural-like cells by coculture with retinal pigmented epithelial cells
AU - Yang, Ling Ling
AU - Zhou, Qing Jun
AU - Wang, Yao
AU - Wang, Yi Qiang
PY - 2010
Y1 - 2010
N2 - AIM: To detect the differentiation effects of retinal cells or extracts on bone marrow-derived mesenchymal stem cells (BMSC). METHODS: Human fetal BMSC were previously labelled by carboxyfluorescein succinimidyl ester (CFSE), and co-cultured with retinal pigment epithelial (RPE) cells which were pre-treated with ultraviolet irradiation at a ratio of 1:1 to induce the differentiation of BMSC for up to 14 days. In some assays, a retinal extract of bovine retinal extract (BRE) was added to detect the potential effects of retinal component on the differentiation of BMSC. In addition, Neuron-specific enolase (NSE), Nestin and Glial fibrillary acidic protein (GFAP) immunostaining were performed to determine the characteristics of BMSC. RESULTS: The results indicated that by co-cultured with RPE cells, fetal BMSC were differentiated into neural-like cells expressing special neuronal markers Nestin, GFAP and NSE. And the expression of these markers was obviously increased by BRE. CONCLUSION: Retina derived cells and extracts can induce the differentiation of BMSC into neural-like cells.
AB - AIM: To detect the differentiation effects of retinal cells or extracts on bone marrow-derived mesenchymal stem cells (BMSC). METHODS: Human fetal BMSC were previously labelled by carboxyfluorescein succinimidyl ester (CFSE), and co-cultured with retinal pigment epithelial (RPE) cells which were pre-treated with ultraviolet irradiation at a ratio of 1:1 to induce the differentiation of BMSC for up to 14 days. In some assays, a retinal extract of bovine retinal extract (BRE) was added to detect the potential effects of retinal component on the differentiation of BMSC. In addition, Neuron-specific enolase (NSE), Nestin and Glial fibrillary acidic protein (GFAP) immunostaining were performed to determine the characteristics of BMSC. RESULTS: The results indicated that by co-cultured with RPE cells, fetal BMSC were differentiated into neural-like cells expressing special neuronal markers Nestin, GFAP and NSE. And the expression of these markers was obviously increased by BRE. CONCLUSION: Retina derived cells and extracts can induce the differentiation of BMSC into neural-like cells.
KW - Bone marrow-derived mesenchymal stem cells
KW - Bovine retinal extract
KW - Neural-like cells
KW - Retinal pigment epithelial cells
UR - http://www.scopus.com/inward/record.url?scp=84892628260&partnerID=8YFLogxK
U2 - 10.3980/j.issn.2222-3959.2010.01.06
DO - 10.3980/j.issn.2222-3959.2010.01.06
M3 - Article
AN - SCOPUS:84892628260
SN - 2222-3959
VL - 3
SP - 23
EP - 27
JO - International Journal of Ophthalmology
JF - International Journal of Ophthalmology
IS - 1
ER -