TY - JOUR
T1 - Differential effects of ethanol in the nucleus accumbens shell of alcohol-preferring (P), alcohol-non-preferring (NP) and Wistar rats
T2 - A proteomics study
AU - McBride, William J.
AU - Schultz, Jonathan A.
AU - Kimpel, Mark W.
AU - McClintick, Jeanette N.
AU - Wang, Mu
AU - You, Jinsam
AU - Rodd, Zachary A.
N1 - Funding Information:
Supported in part by AA07611, AA016652 [INIA project], AA013522 [INIA project] and INGEN ® (which is partially funded by Lilly Endowment Inc.).
PY - 2009/4
Y1 - 2009/4
N2 - The objective of this study was to determine the effects of ethanol injections on protein expression in the nucleus accumbens shell (ACB-sh) of alcohol-preferring (P), alcohol-non-preferring (NP) and Wistar (W) rats. Rats were injected for 5 consecutive days with either saline or 1 g/kg ethanol; 24 h after the last injection, rats were killed and brains obtained. Micro-punch samples of the ACB-sh were homogenized; extracted proteins were subjected to trypsin digestion and analyzed with a liquid chromatography-mass spectrometer procedure. Ethanol changed expression levels (1.15-fold or higher) of 128 proteins in NP rats, 22 proteins in P, and 28 proteins in W rats. Few of the changes observed with ethanol treatment for NP rats were observed for P and W rats. Many of the changes occurred in calcium-calmodulin signaling systems, G-protein signaling systems, synaptic structure and histones. Approximately half the changes observed in the ACB-sh of P rats were also observed for W rats. Overall, the results indicate a unique response to ethanol of the ACB-sh of NP rats compared to P and W rats; this unique response may reflect changes in neuronal function in the ACB-sh that could contribute to the low alcohol drinking behavior of the NP line.
AB - The objective of this study was to determine the effects of ethanol injections on protein expression in the nucleus accumbens shell (ACB-sh) of alcohol-preferring (P), alcohol-non-preferring (NP) and Wistar (W) rats. Rats were injected for 5 consecutive days with either saline or 1 g/kg ethanol; 24 h after the last injection, rats were killed and brains obtained. Micro-punch samples of the ACB-sh were homogenized; extracted proteins were subjected to trypsin digestion and analyzed with a liquid chromatography-mass spectrometer procedure. Ethanol changed expression levels (1.15-fold or higher) of 128 proteins in NP rats, 22 proteins in P, and 28 proteins in W rats. Few of the changes observed with ethanol treatment for NP rats were observed for P and W rats. Many of the changes occurred in calcium-calmodulin signaling systems, G-protein signaling systems, synaptic structure and histones. Approximately half the changes observed in the ACB-sh of P rats were also observed for W rats. Overall, the results indicate a unique response to ethanol of the ACB-sh of NP rats compared to P and W rats; this unique response may reflect changes in neuronal function in the ACB-sh that could contribute to the low alcohol drinking behavior of the NP line.
KW - Alcohol
KW - Alcohol-non-preferring rats
KW - Alcohol-preferring rats
KW - Nucleus accumbens shell
KW - Protein expression
KW - Proteomics
UR - http://www.scopus.com/inward/record.url?scp=59649096975&partnerID=8YFLogxK
U2 - 10.1016/j.pbb.2008.12.019
DO - 10.1016/j.pbb.2008.12.019
M3 - Article
C2 - 19166871
AN - SCOPUS:59649096975
SN - 0091-3057
VL - 92
SP - 304
EP - 313
JO - Pharmacology Biochemistry and Behavior
JF - Pharmacology Biochemistry and Behavior
IS - 2
ER -