Development of a smart packaging for the monitoring of fish spoilage

There is much interest from the fisheries industry in developing rapid methods to evaluate real-time freshness of fish and seafood products. Emphasis is on the ones that would reflect and account for the products history and their storage conditions from "harvest-to-home". The development of a "smart packaging" that monitors the microbial breakdown products in the headspace of packaged fish is described. When fish spoils it releases a variety of basic volatile amines which are detectable with appropriate pH indicating sensors. These are prepared by entrapping within a polymer matrix a pH sensitive dye that responds, through visible color changes to the spoilage volatile compounds that contribute to a quantity known as total volatile basic nitrogen (TVB-N). Laboratory trials on fresh fish filets showed that the sensor accurately tracks the increase in amines concentration in the package headspace. The response was also found to correlate to changing microbial populations (total viable count or TVC and Pseudomonas spp.). In addition, leaching of the dye was assessed over time to assess the suitability of the sensor formulation for food packaging application.