TY - GEN
T1 - Detection of nitrite by flow injection analysis using a novel Paired Emitter-detector Diode (PEDD) as a photometric detector
AU - O'Toole, Martina
AU - Shepherd, Roderick
AU - Lau, King Tong
AU - Diamond, Dermot
N1 - Funding Information:
The authors would like to thank Dr. David Lazer, Iolanthe Chronis and various undergraduate contributors for their help with the experimental deployment. Anmol Madan and Alex Pentland were partially sponsored by the Army Research Laboratory under Cooperative Agreement Number W911NF-09-2-0053, and by AFOSR under Award Number FA9550-10-1-0122. Views and conclusions in this document are those of the authors and should not be interpreted as representing the official policies, either expressed or implied, of the Army Research Laboratory or the U.S. Government. The U.S. Government is authorized to reproduce and distribute reprints for Government purposes notwithstanding any copyright notation. Katayoun Farrahi and Daniel Gatica-Perez were supported by the Swiss National Science Foundation through the MULTI and HAI projects.
PY - 2007
Y1 - 2007
N2 - An inexpensive flow injection analysis system for determining low concentration levels of nitrite employing the Griess reagent spectrophotometric method is reported. The novel photometric detector applied within this manifold is a highly sensitive, low cost, miniaturized light emitting diode (LED) based flow detector. This colorimetric detector employs two LEDs, operating one as a light source and the other as a light detector. The emitter LED is forward biased and the detector reverse biased. The emitter and detector LED had a λmax of 530 nm and 623 nm respectively. The emission spectrum of the emitter LED efficiently overlapped with the absorbance spectrum of 9 μM NO2 and Griess reagent complex. A simple timer circuit measures the time taken for the photocurrent generated by the emitter LED to discharge the detector LED from 5 V (logic 1) to 1.7 V (logic 0). The Griess reagent method employed for nitrite determination is based on the formation of an azo dye, the intensity of which is directly related to nitrite concentration. The linear range, reproducibility and limit of detection were investigated. Detection limits in the nanomolar range were achieved using the Paired Emitter-Detector Diode (PEDD) flow analysis device. For a comparative study the linear range and limit of detection were also investigated using a platewell reader. Higher sensitivity and improved precision were obtained from the PEDD compared to the commercially available plate well reader.
AB - An inexpensive flow injection analysis system for determining low concentration levels of nitrite employing the Griess reagent spectrophotometric method is reported. The novel photometric detector applied within this manifold is a highly sensitive, low cost, miniaturized light emitting diode (LED) based flow detector. This colorimetric detector employs two LEDs, operating one as a light source and the other as a light detector. The emitter LED is forward biased and the detector reverse biased. The emitter and detector LED had a λmax of 530 nm and 623 nm respectively. The emission spectrum of the emitter LED efficiently overlapped with the absorbance spectrum of 9 μM NO2 and Griess reagent complex. A simple timer circuit measures the time taken for the photocurrent generated by the emitter LED to discharge the detector LED from 5 V (logic 1) to 1.7 V (logic 0). The Griess reagent method employed for nitrite determination is based on the formation of an azo dye, the intensity of which is directly related to nitrite concentration. The linear range, reproducibility and limit of detection were investigated. Detection limits in the nanomolar range were achieved using the Paired Emitter-Detector Diode (PEDD) flow analysis device. For a comparative study the linear range and limit of detection were also investigated using a platewell reader. Higher sensitivity and improved precision were obtained from the PEDD compared to the commercially available plate well reader.
KW - Griess reaction and flow analysis
KW - Light emitting diodes
KW - Nitrite detection
KW - Optical sensing
UR - http://www.scopus.com/inward/record.url?scp=42449084254&partnerID=8YFLogxK
U2 - 10.1117/12.737791
DO - 10.1117/12.737791
M3 - Conference Proceeding
AN - SCOPUS:42449084254
SN - 9780819469151
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Advanced Environmental, Chemical, and Biological Sensing Technologies V
T2 - Advanced Environmental, Chemical, and Biological Sensing Technologies V
Y2 - 10 September 2007 through 11 September 2007
ER -