TY - JOUR
T1 - Characterization of behavioral and endocrine effects of LSD on zebrafish
AU - Grossman, Leah
AU - Utterback, Eli
AU - Stewart, Adam
AU - Gaikwad, Siddharth
AU - Chung, Kyung Min
AU - Suciu, Christopher
AU - Wong, Keith
AU - Elegante, Marco
AU - Elkhayat, Salem
AU - Tan, Julia
AU - Gilder, Thomas
AU - Wu, Nadine
AU - DiLeo, John
AU - Cachat, Jonathan
AU - Kalueff, Allan V.
PY - 2010/12
Y1 - 2010/12
N2 - Lysergic acid diethylamide (LSD) is a potent hallucinogenic drug that strongly affects animal and human behavior. Although adult zebrafish (Danio rerio) are emerging as a promising neurobehavioral model, the effects of LSD on zebrafish have not been investigated previously. Several behavioral paradigms (the novel tank, observation cylinder, light-dark box, open field, T-maze, social preference and shoaling tests), as well as modern video-tracking tools and whole-body cortisol assay were used to characterize the effects of acute LSD in zebrafish. While lower doses (5-100μg/L) did not affect zebrafish behavior, 250μg/L LSD increased top dwelling and reduced freezing in the novel tank and observation cylinder tests, also affecting spatiotemporal patterns of activity (as assessed by 3D reconstruction of zebrafish traces and ethograms). LSD evoked mild thigmotaxis in the open field test, increased light behavior in the light-dark test, reduced the number of arm entries and freezing in the T-maze and social preference test, without affecting social preference. In contrast, LSD affected zebrafish shoaling (increasing the inter-fish distance in a group), and elevated whole-body cortisol levels. Overall, our findings show sensitivity of zebrafish to LSD action, and support the use of zebrafish models to study hallucinogenic drugs of abuse.
AB - Lysergic acid diethylamide (LSD) is a potent hallucinogenic drug that strongly affects animal and human behavior. Although adult zebrafish (Danio rerio) are emerging as a promising neurobehavioral model, the effects of LSD on zebrafish have not been investigated previously. Several behavioral paradigms (the novel tank, observation cylinder, light-dark box, open field, T-maze, social preference and shoaling tests), as well as modern video-tracking tools and whole-body cortisol assay were used to characterize the effects of acute LSD in zebrafish. While lower doses (5-100μg/L) did not affect zebrafish behavior, 250μg/L LSD increased top dwelling and reduced freezing in the novel tank and observation cylinder tests, also affecting spatiotemporal patterns of activity (as assessed by 3D reconstruction of zebrafish traces and ethograms). LSD evoked mild thigmotaxis in the open field test, increased light behavior in the light-dark test, reduced the number of arm entries and freezing in the T-maze and social preference test, without affecting social preference. In contrast, LSD affected zebrafish shoaling (increasing the inter-fish distance in a group), and elevated whole-body cortisol levels. Overall, our findings show sensitivity of zebrafish to LSD action, and support the use of zebrafish models to study hallucinogenic drugs of abuse.
KW - Adult zebrafish
KW - Behavioral paradigms
KW - Cortisol
KW - Hallucinogenic drugs
KW - LSD
KW - Screening
UR - http://www.scopus.com/inward/record.url?scp=77955266291&partnerID=8YFLogxK
U2 - 10.1016/j.bbr.2010.05.039
DO - 10.1016/j.bbr.2010.05.039
M3 - Article
C2 - 20561961
AN - SCOPUS:77955266291
SN - 0166-4328
VL - 214
SP - 277
EP - 284
JO - Behavioural Brain Research
JF - Behavioural Brain Research
IS - 2
ER -