TY - JOUR
T1 - Biocompatibility and mechanical properties of pigeon bone waste extracted natural nano-hydroxyapatite for bone tissue engineering
AU - Sharifianjazi, Fariborz
AU - Esmaeilkhanian, Amirhossein
AU - Moradi, Mostafa
AU - Pakseresht, Amirhosein
AU - Asl, Mehdi Shahedi
AU - Karimi-Maleh, Hassan
AU - Jang, Ho Won
AU - Shokouhimehr, Mohammadreza
AU - Varma, Rajender S.
N1 - Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2021/2
Y1 - 2021/2
N2 - One of the common bioactive materials used for clinical and biomedical applications is hydroxyapatite (HAp). Bio-waste materials are one of the major natural sources for the preparation of this bio-ceramic powder. Herein, naturally derived nano-HAp was prepared using the ball milling process after annealing of waste pigeon bones at 850 °C followed by cold-pressing the nanoparticles and re-sintering at 850, 950, 1050, and 1150 °C. The ball-milled pigeon-derived nano-hydroxyapatite (PHA) had an average particle size in the range of 50–250 nm and the Ca/P ratio of the sample sintered at 1050 °C was 1.7. Moreover, the hardness and compressive strength of sintered nano-HAp were improved to 47.57 MPa and 3.7 GPa, respectively by increasing the sintering temperature. Furthermore, alkaline phosphatase analysis and MTT assay of PHA indicated significant enhancement in the activity and proliferation of osteoblast cells during the culturing period in comparison to synthetic HAp.
AB - One of the common bioactive materials used for clinical and biomedical applications is hydroxyapatite (HAp). Bio-waste materials are one of the major natural sources for the preparation of this bio-ceramic powder. Herein, naturally derived nano-HAp was prepared using the ball milling process after annealing of waste pigeon bones at 850 °C followed by cold-pressing the nanoparticles and re-sintering at 850, 950, 1050, and 1150 °C. The ball-milled pigeon-derived nano-hydroxyapatite (PHA) had an average particle size in the range of 50–250 nm and the Ca/P ratio of the sample sintered at 1050 °C was 1.7. Moreover, the hardness and compressive strength of sintered nano-HAp were improved to 47.57 MPa and 3.7 GPa, respectively by increasing the sintering temperature. Furthermore, alkaline phosphatase analysis and MTT assay of PHA indicated significant enhancement in the activity and proliferation of osteoblast cells during the culturing period in comparison to synthetic HAp.
KW - Bio-waste materials
KW - Biocompatibility
KW - Bone
KW - Compressive strength
KW - Nano-hydroxyapatite
KW - Nanomaterials
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=85097877361&partnerID=8YFLogxK
U2 - 10.1016/j.mseb.2020.114950
DO - 10.1016/j.mseb.2020.114950
M3 - Article
AN - SCOPUS:85097877361
SN - 0921-5107
VL - 264
JO - Materials Science and Engineering: B
JF - Materials Science and Engineering: B
M1 - 114950
ER -