TY - JOUR
T1 - Behavioral and physiological effects of RDX on adult zebrafish
AU - Williams, Larry R.
AU - Wong, Keith
AU - Stewart, Adam
AU - Suciu, Christopher
AU - Gaikwad, Siddharth
AU - Wu, Nadine
AU - Dileo, John
AU - Grossman, Leah
AU - Cachat, Jonathan
AU - Hart, Peter
AU - Kalueff, Allan V.
PY - 2012/1
Y1 - 2012/1
N2 - 1,3,5-Trinitroperhydro-1,3,5-triazine (RDX) is a nitroamine explosive, with common toxic effects including seizures. Here, we explore the behavioral effects of acute RDX exposure in adult zebrafish Danio rerio, a rapidly developing model in neuroscience and neurotoxicology research. Overall, a 30-min exposure to RDX low dose of 0.1 mM evoked behavioral activation in zebrafish, while a higher dose of 1 mM markedly reduced exploration, increased freezing and evoked seizure-like responses (i.e., bouts of hyperactivity, spasms, and corkscrew swimming). Likewise, whole-body cortisol levels were also significantly elevated in fish exposed to 1 mM (but not 0.1 mM) RDX. In line with clinical and animal data, our study demonstrates the dose-dependent behavioral activation and pro-convulsant effects of RDX in zebrafish-based models.
AB - 1,3,5-Trinitroperhydro-1,3,5-triazine (RDX) is a nitroamine explosive, with common toxic effects including seizures. Here, we explore the behavioral effects of acute RDX exposure in adult zebrafish Danio rerio, a rapidly developing model in neuroscience and neurotoxicology research. Overall, a 30-min exposure to RDX low dose of 0.1 mM evoked behavioral activation in zebrafish, while a higher dose of 1 mM markedly reduced exploration, increased freezing and evoked seizure-like responses (i.e., bouts of hyperactivity, spasms, and corkscrew swimming). Likewise, whole-body cortisol levels were also significantly elevated in fish exposed to 1 mM (but not 0.1 mM) RDX. In line with clinical and animal data, our study demonstrates the dose-dependent behavioral activation and pro-convulsant effects of RDX in zebrafish-based models.
KW - Behavioral activation
KW - Cortisol
KW - Epilepsy
KW - Pro-convulsant action
KW - Seizures
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=81855175313&partnerID=8YFLogxK
U2 - 10.1016/j.cbpc.2011.02.010
DO - 10.1016/j.cbpc.2011.02.010
M3 - Article
C2 - 21382508
AN - SCOPUS:81855175313
SN - 1532-0456
VL - 155
SP - 33
EP - 38
JO - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
JF - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
IS - 1
ER -