TY - JOUR
T1 - Behavioral and physiological effects of acute ketamine exposure in adult zebrafish
AU - Riehl, Russell
AU - Kyzar, Evan
AU - Allain, Alexander
AU - Green, Jeremy
AU - Hook, Molly
AU - Monnig, Louis
AU - Rhymes, Kate
AU - Roth, Andrew
AU - Pham, Mimi
AU - Razavi, Roshan
AU - DiLeo, John
AU - Gaikwad, Siddharth
AU - Hart, Peter
AU - Kalueff, Allan V.
PY - 2011/11
Y1 - 2011/11
N2 - Ketamine is a non-competitive glutamatergic antagonist used to induce sedation and analgesia. In sub-anesthetic doses, it induces hyperlocomotion, impairs memory and evokes stereotypic circling in rodents. Zebrafish (Danio rerio) emerged as a promising new animal model to screen the effects of psychotropic compounds. Here, we investigated the effects of sub-anesthetic doses of ketamine on anxiety, locomotion, habituation and social behavior of adult zebrafish. Acute 20-min exposure to 20 and 40. mg/L (but not 2. mg/L) of ketamine reduced anxiety, impaired intra-session habituation, evoked circular swimming and disrupted zebrafish shoaling. Additionally, ketamine reduced whole-body cortisol levels and elevated brain c-fos expression in zebrafish. Our findings demonstrate the sensitivity of zebrafish to behavioral and physiological effects of sub-anesthetic doses of ketamine, further supporting the utility of this species as a model for neuropharmacological research, including testing ketamine and related drugs.
AB - Ketamine is a non-competitive glutamatergic antagonist used to induce sedation and analgesia. In sub-anesthetic doses, it induces hyperlocomotion, impairs memory and evokes stereotypic circling in rodents. Zebrafish (Danio rerio) emerged as a promising new animal model to screen the effects of psychotropic compounds. Here, we investigated the effects of sub-anesthetic doses of ketamine on anxiety, locomotion, habituation and social behavior of adult zebrafish. Acute 20-min exposure to 20 and 40. mg/L (but not 2. mg/L) of ketamine reduced anxiety, impaired intra-session habituation, evoked circular swimming and disrupted zebrafish shoaling. Additionally, ketamine reduced whole-body cortisol levels and elevated brain c-fos expression in zebrafish. Our findings demonstrate the sensitivity of zebrafish to behavioral and physiological effects of sub-anesthetic doses of ketamine, further supporting the utility of this species as a model for neuropharmacological research, including testing ketamine and related drugs.
KW - Anxiety
KW - Aquatic models
KW - Behavioral testing
KW - C-fos expression
KW - Cortisol
KW - Locomotion and circling
UR - http://www.scopus.com/inward/record.url?scp=84855955477&partnerID=8YFLogxK
U2 - 10.1016/j.ntt.2011.05.011
DO - 10.1016/j.ntt.2011.05.011
M3 - Article
C2 - 21683787
AN - SCOPUS:84855955477
SN - 0892-0362
VL - 33
SP - 658
EP - 667
JO - Neurotoxicology and Teratology
JF - Neurotoxicology and Teratology
IS - 6
ER -