Antigen/Antibody Immunocomplex from CdTe Nanoparticle Bioconjugates

Shaopeng Wang; Natalia Mamedova; Nicholas A. Kotov; Wei Chen; Joe Studer

doi:10.1021/nl0255193

Antigen/Antibody Immunocomplex from CdTe Nanoparticle Bioconjugates

Shaopeng Wang^*, Natalia Mamedova, Nicholas A. Kotov, Wei Chen, Joe Studer

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

571 Citations (Scopus)

Abstract

Complementary bioconjugates based on antibody-antigen interactions were synthesized from luminescent CdTe nanoparticles (NPs). Antigen (bovine serum albumin) was conjugated to red-emitting CdTe NPs, while green-emitting NPs were attached to the corresponding anti-BSA antibody (IgG). The NP bioconjugates were characterized by native and SDS-PAGE electrophoresis, gel-permeation HPLC, and circular dichroism. Antigen-antibody binding affinity was evaluated by enzyme-linked immunosorbent assay (ELISA). The formation of BSA-IgG immunocomplex resulted in the Förster resonance energy transfer (FRET) between the two different NPs: the luminescence of green-emitting NPs was quenched whereas the emission of the red-emitting NPs was enhanced. The luminescence recovered when the immunocomplex was exposed to an unlabeled antigen. The immunocomplexes can be considered as a prototype of NP superstructures based on biospecific ligands, while the competitive FRET inhibition can be used in an immunoassay protocol.

Original language	English
Pages (from-to)	817-822
Number of pages	6
Journal	Nano Letters
Volume	2
Issue number	8
DOIs	https://doi.org/10.1021/nl0255193
Publication status	Published - Aug 2002
Externally published	Yes

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title = "Antigen/Antibody Immunocomplex from CdTe Nanoparticle Bioconjugates",

abstract = "Complementary bioconjugates based on antibody-antigen interactions were synthesized from luminescent CdTe nanoparticles (NPs). Antigen (bovine serum albumin) was conjugated to red-emitting CdTe NPs, while green-emitting NPs were attached to the corresponding anti-BSA antibody (IgG). The NP bioconjugates were characterized by native and SDS-PAGE electrophoresis, gel-permeation HPLC, and circular dichroism. Antigen-antibody binding affinity was evaluated by enzyme-linked immunosorbent assay (ELISA). The formation of BSA-IgG immunocomplex resulted in the F{\"o}rster resonance energy transfer (FRET) between the two different NPs: the luminescence of green-emitting NPs was quenched whereas the emission of the red-emitting NPs was enhanced. The luminescence recovered when the immunocomplex was exposed to an unlabeled antigen. The immunocomplexes can be considered as a prototype of NP superstructures based on biospecific ligands, while the competitive FRET inhibition can be used in an immunoassay protocol.",

author = "Shaopeng Wang and Natalia Mamedova and Kotov, {Nicholas A.} and Wei Chen and Joe Studer",

year = "2002",

month = aug,

doi = "10.1021/nl0255193",

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T1 - Antigen/Antibody Immunocomplex from CdTe Nanoparticle Bioconjugates

AU - Wang, Shaopeng

AU - Mamedova, Natalia

AU - Kotov, Nicholas A.

AU - Chen, Wei

AU - Studer, Joe

PY - 2002/8

Y1 - 2002/8

N2 - Complementary bioconjugates based on antibody-antigen interactions were synthesized from luminescent CdTe nanoparticles (NPs). Antigen (bovine serum albumin) was conjugated to red-emitting CdTe NPs, while green-emitting NPs were attached to the corresponding anti-BSA antibody (IgG). The NP bioconjugates were characterized by native and SDS-PAGE electrophoresis, gel-permeation HPLC, and circular dichroism. Antigen-antibody binding affinity was evaluated by enzyme-linked immunosorbent assay (ELISA). The formation of BSA-IgG immunocomplex resulted in the Förster resonance energy transfer (FRET) between the two different NPs: the luminescence of green-emitting NPs was quenched whereas the emission of the red-emitting NPs was enhanced. The luminescence recovered when the immunocomplex was exposed to an unlabeled antigen. The immunocomplexes can be considered as a prototype of NP superstructures based on biospecific ligands, while the competitive FRET inhibition can be used in an immunoassay protocol.

AB - Complementary bioconjugates based on antibody-antigen interactions were synthesized from luminescent CdTe nanoparticles (NPs). Antigen (bovine serum albumin) was conjugated to red-emitting CdTe NPs, while green-emitting NPs were attached to the corresponding anti-BSA antibody (IgG). The NP bioconjugates were characterized by native and SDS-PAGE electrophoresis, gel-permeation HPLC, and circular dichroism. Antigen-antibody binding affinity was evaluated by enzyme-linked immunosorbent assay (ELISA). The formation of BSA-IgG immunocomplex resulted in the Förster resonance energy transfer (FRET) between the two different NPs: the luminescence of green-emitting NPs was quenched whereas the emission of the red-emitting NPs was enhanced. The luminescence recovered when the immunocomplex was exposed to an unlabeled antigen. The immunocomplexes can be considered as a prototype of NP superstructures based on biospecific ligands, while the competitive FRET inhibition can be used in an immunoassay protocol.

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JO - Nano Letters

JF - Nano Letters

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ER -

Antigen/Antibody Immunocomplex from CdTe Nanoparticle Bioconjugates

Abstract

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