TY - JOUR
T1 - Antibiotic Toxicity Profiles of Escherichia coli Strains Lacking DNA Methyltransferases
AU - Chen, Zheng
AU - Wang, Hailin
N1 - Funding Information:
This work was supported by grants from the National Natural Science Foundation of China (21927807, 91743201, and 22021003) and the Ministry of Science and Technology of China (2018YFC1005003 and Y9L10301) to H.W.
Publisher Copyright:
© 2021 The Authors. Published by American Chemical Society.
PY - 2021/3/23
Y1 - 2021/3/23
N2 - Antibiotic-resistant bacteria are causing more antibiotic treatment failures. Developing new antibiotics and identifying bacterial targets will help to mitigate the emergence and reduce the spread of antibiotic resistance in the environment. We investigated whether DNA methyltransferase (MTase) can be an adjunct target for improving antibiotic toxicity. We used Escherichia coli as an example. The genes encoding DNA adenine MTase and cytosine MTase, dam and dcm, respectively, were separately knocked out using the λRed system in E. coli MG1655. MG1655 and the two knockout strains were separately exposed in 96-well plates to 20 antibiotics from five classes. The EC50 values of almost all of the tested antibiotics were lower in the dam and dcm knockout lines than that of the control. Our statistical analysis showed that the variations observed in EC50 values were independent of the mechanism underlying each antibiotic's mechanistic action.
AB - Antibiotic-resistant bacteria are causing more antibiotic treatment failures. Developing new antibiotics and identifying bacterial targets will help to mitigate the emergence and reduce the spread of antibiotic resistance in the environment. We investigated whether DNA methyltransferase (MTase) can be an adjunct target for improving antibiotic toxicity. We used Escherichia coli as an example. The genes encoding DNA adenine MTase and cytosine MTase, dam and dcm, respectively, were separately knocked out using the λRed system in E. coli MG1655. MG1655 and the two knockout strains were separately exposed in 96-well plates to 20 antibiotics from five classes. The EC50 values of almost all of the tested antibiotics were lower in the dam and dcm knockout lines than that of the control. Our statistical analysis showed that the variations observed in EC50 values were independent of the mechanism underlying each antibiotic's mechanistic action.
UR - http://www.scopus.com/inward/record.url?scp=85103794625&partnerID=8YFLogxK
U2 - 10.1021/acsomega.1c00378
DO - 10.1021/acsomega.1c00378
M3 - Article
AN - SCOPUS:85103794625
SN - 2470-1343
VL - 6
SP - 7834
EP - 7840
JO - ACS Omega
JF - ACS Omega
IS - 11
ER -