An LC-QToF MS based method for untargeted metabolomics of human fecal samples

Ken Cheng; Carl Brunius; Rikard Fristedt; Rikard Landberg

doi:10.1007/s11306-020-01669-z

An LC-QToF MS based method for untargeted metabolomics of human fecal samples

Ken Cheng^*, Carl Brunius, Rikard Fristedt, Rikard Landberg

^*Corresponding author for this work

Chalmers University of Technology

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

Introduction: Consensus in sample preparation for untargeted human fecal metabolomics is lacking. Objectives: To obtain sample preparation with broad metabolite coverage for high-throughput LC–MS. Methods: Extraction solvent, solvent ratio and fresh frozen-vs-lyophilized samples were evaluated by metabolite feature quality. Results: Methanol at 5 mL per g wet feces provided a wide metabolite coverage with optimal balance between signal intensity and saturation for both fresh frozen and lyophilized samples. Lyophilization did not affect SCFA and is recommended because of convenience in normalizing to dry matter. Conclusion: The suggested sample preparation is simple, efficient and suitable for large-scale human fecal metabolomics.

Original language	English
Article number	46
Journal	Metabolomics
Volume	16
Issue number	4
DOIs	https://doi.org/10.1007/s11306-020-01669-z
Publication status	Published - 1 Apr 2020
Externally published	Yes

Keywords

Fecal sample
Freeze-drying
Method optimization
Short-chain fatty acids
Untargeted fecal metabolomics
XCMS

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s11306-020-01669-z

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abstract = "Introduction: Consensus in sample preparation for untargeted human fecal metabolomics is lacking. Objectives: To obtain sample preparation with broad metabolite coverage for high-throughput LC–MS. Methods: Extraction solvent, solvent ratio and fresh frozen-vs-lyophilized samples were evaluated by metabolite feature quality. Results: Methanol at 5 mL per g wet feces provided a wide metabolite coverage with optimal balance between signal intensity and saturation for both fresh frozen and lyophilized samples. Lyophilization did not affect SCFA and is recommended because of convenience in normalizing to dry matter. Conclusion: The suggested sample preparation is simple, efficient and suitable for large-scale human fecal metabolomics.",

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doi = "10.1007/s11306-020-01669-z",

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T1 - An LC-QToF MS based method for untargeted metabolomics of human fecal samples

AU - Cheng, Ken

AU - Brunius, Carl

AU - Fristedt, Rikard

AU - Landberg, Rikard

PY - 2020/4/1

Y1 - 2020/4/1

N2 - Introduction: Consensus in sample preparation for untargeted human fecal metabolomics is lacking. Objectives: To obtain sample preparation with broad metabolite coverage for high-throughput LC–MS. Methods: Extraction solvent, solvent ratio and fresh frozen-vs-lyophilized samples were evaluated by metabolite feature quality. Results: Methanol at 5 mL per g wet feces provided a wide metabolite coverage with optimal balance between signal intensity and saturation for both fresh frozen and lyophilized samples. Lyophilization did not affect SCFA and is recommended because of convenience in normalizing to dry matter. Conclusion: The suggested sample preparation is simple, efficient and suitable for large-scale human fecal metabolomics.

AB - Introduction: Consensus in sample preparation for untargeted human fecal metabolomics is lacking. Objectives: To obtain sample preparation with broad metabolite coverage for high-throughput LC–MS. Methods: Extraction solvent, solvent ratio and fresh frozen-vs-lyophilized samples were evaluated by metabolite feature quality. Results: Methanol at 5 mL per g wet feces provided a wide metabolite coverage with optimal balance between signal intensity and saturation for both fresh frozen and lyophilized samples. Lyophilization did not affect SCFA and is recommended because of convenience in normalizing to dry matter. Conclusion: The suggested sample preparation is simple, efficient and suitable for large-scale human fecal metabolomics.

KW - Fecal sample

KW - Freeze-drying

KW - Method optimization

KW - Short-chain fatty acids

KW - Untargeted fecal metabolomics

KW - XCMS

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JO - Metabolomics

JF - Metabolomics

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ER -

An LC-QToF MS based method for untargeted metabolomics of human fecal samples

Abstract

Keywords

UN SDGs

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