TY - JOUR
T1 - Age-related defects in erythrocyte 2,3-diphosphoglycerate metabolism in dementia
AU - Kaminsky, Yury G.
AU - Prakash Reddy, V.
AU - Ashraf, Ghulam Md
AU - Ahmad, Ausaf
AU - Benberin, Valery V.
AU - Kosenko, Elena A.
AU - Aliev, Gjumrakch
PY - 2013
Y1 - 2013
N2 - Alzheimer disease (AD) is the most common dementing illness. Metabolic defects in the brain with aging contribute to the pathogenesis of AD. These changes can be found systematically and thus can be used as potential biomarkers. Erythrocytes (RBCs) are passive "reporter cells" that are not well studied in AD. In the present study, we analyzed an array of glycolytic and related enzymes and intermediates in RBCs from patients with AD and non-Alzheimer dementia (NA), age-matched controls (AC) and young adult controls (YC). AD is characterized by higher activities of hexokinase, phosphofructokinase, and bisphosphoglycerate mutase and bisphosphoglycerate phosphatase in RBCs. In our study, we observed that glycolytic and related enzymes displayed significantly lower activities in AC. However, similar or significantly higher activities were observed in AD and NA groups as compared to YC group. 2,3-diphosphoglycerate (2,3-DPG) levels were significantly decreased in AD and NA patients. The pattern of changes between groups in the above indices strongly correlates with each other. Collectively, our data suggested that AD and NA patients are associated with chronic disturbance of 2,3-DPG metabolism in RBCs. These defects may play a pivotal role in physiological processes, which predispose elderly subjects to AD and NA.
AB - Alzheimer disease (AD) is the most common dementing illness. Metabolic defects in the brain with aging contribute to the pathogenesis of AD. These changes can be found systematically and thus can be used as potential biomarkers. Erythrocytes (RBCs) are passive "reporter cells" that are not well studied in AD. In the present study, we analyzed an array of glycolytic and related enzymes and intermediates in RBCs from patients with AD and non-Alzheimer dementia (NA), age-matched controls (AC) and young adult controls (YC). AD is characterized by higher activities of hexokinase, phosphofructokinase, and bisphosphoglycerate mutase and bisphosphoglycerate phosphatase in RBCs. In our study, we observed that glycolytic and related enzymes displayed significantly lower activities in AC. However, similar or significantly higher activities were observed in AD and NA groups as compared to YC group. 2,3-diphosphoglycerate (2,3-DPG) levels were significantly decreased in AD and NA patients. The pattern of changes between groups in the above indices strongly correlates with each other. Collectively, our data suggested that AD and NA patients are associated with chronic disturbance of 2,3-DPG metabolism in RBCs. These defects may play a pivotal role in physiological processes, which predispose elderly subjects to AD and NA.
KW - 2,3-diphosphoglycerate
KW - Erythrocyte
KW - Glycolysis
KW - Late onset Alzheimer disease
KW - Non-Alzheimer's dementia
KW - Oxygen affinity of hemoglobin
UR - http://www.scopus.com/inward/record.url?scp=84896700209&partnerID=8YFLogxK
U2 - 10.14336/AD.2013.0400244
DO - 10.14336/AD.2013.0400244
M3 - Article
AN - SCOPUS:84896700209
SN - 2152-5250
VL - 4
SP - 244
EP - 255
JO - Aging and Disease
JF - Aging and Disease
IS - 5
ER -